Difference between revisions of "IGEM Notebook (6/2/09 - 7/31/09)"

Revision as of 12:57, 5 June 2009

--Leland 09:28, 2 June 2009 (EDT)

Today Alyndria and I worked on answering the question of how do we insert and construct the logical clauses (LCs) into the reporter gene. We outlined two processes for doing the LC inserts. One as a BioBrick, and the other only changing the first part of the gene. We feel the second one is much better because there are only two parts (of the gene) to be linked together, and only the first part has to be edited.

Tomorrow Todo List
1. Finalize and upload the document created today
2. Begin work on which tRNA suppressor we should use.

--Leland 17:35, 3 June 2009 (EDT)

I worked on putting together a document showing which tRNAs we should use. Will came up with an alternative method for how we will insert the logical clauses (or anything); however, we have yet to create a document showing this method. We went ahead and ordered all the tRNAs except for the wobble one (11 total), so that we can begin testing them. I put together the ATG and 5mer sequences for preliminary tests on our system. These sequences will be used to test to see if our idea of having one suppressor tRNA bind to mRNA and alter the reading frame, allowing a protein to be expressed, will work. Davidson ATG+5mer BioBrick Sequences

Tomorrow Todo List
1. Finalize and upload the document on which tRNA suppressor we should use
2. Check the ATG+5mer sequences with the reporter genes with Shashank
3. Design the other surpressor tRNA encoding biobrick
4. Update the document created yesterday with the new plan

--Leland 10:13, 4 June 2009 (EDT)

Went through RFP and Tet resistance. No 5mer show up in reading frame (good). Updated tRNA doc. Inserting 5mers

Tomorrow Todo List
1. Choose a reporter gene... needs to have a restriction site early on 2. Work out ideas to overcome TAG (UAG) in reading frame 3. Finish Question