Difference between revisions of "KCM Preparation"
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*1.5 mL - 1M CaCl<sub>2</sub> | *1.5 mL - 1M CaCl<sub>2</sub> | ||
*3.5 mL - H<sub>2</sub>O | *3.5 mL - H<sub>2</sub>O | ||
| − | + | ||
10X | 10X | ||
Filter sterilize (0.22 μm filter) | Filter sterilize (0.22 μm filter) | ||
Revision as of 23:24, 30 October 2008
- 2.5 mL - 2M KCl
- 2.5 mL - 1M MgCl2
- 1.5 mL - 1M CaCl2
- 3.5 mL - H2O
10X
Filter sterilize (0.22 μm filter)
