Laura Voss - Synthetic Biology Seminar
From GcatWiki
Contents
Paper Discussion 10/23
Links for "Relative Sensitivities of Various Reporter Genes"
- Ice Nucleation Proteins and inaZ. InaZ is a reporter for ice nucleation proteins, which, according to NCBI, "enable bacteria to nucleate crystallation in supercooled water". Since supercooled water is chilled below its freezing point, ice nucleation proteins form the "seed" around which ice crystals can form in the cell. The more proteins are produced, the more ice crystals will be present in a cell. See the Wikipedia entry on supercooling.
- Sugars used to test promoter's responsiveness to sucrose. Several sugars were tested with the sucrose-responsive promoter to ensure its specificity. Sucrose, fructose, and sorbose were among the sugars tested, and were the only ones that elicited a response from the promoter. Illustrations of their molecular structure are in the next page.
Links for "Simulation of GFP Expression"
- Michaelis-Menten Kinetics is a system of equations that describes the kinetics of enzymes when the concentration of substrate is much greater than the concentration of enzyme. In this paper, Michaelis-Menten kinetics were used to describe the degradation of GFP, or, more accurately, the activity of the protease responsible for the degradation of GFP.
Class Wiki Project
My class project will be on the design of temperature-sensing cellular mechanisms, such as cellular thermometers.
