Difference between revisions of "M13 Bacteriophage Production Protocol"
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| − | + | 1. Grow 3mL of cells with phage plasmid in liquid media overnight. These cells will produce M13 phage which can then be isolated. For example, grow J100265 + J100270 S1030 cells in 50ug/mLCarb + 50ug/mL Kan + 10ug/mL Tet for SPT7-spec phage. | |
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| + | 2. Centrifuge 1.5mL of cells at full speed (~15,500 RPM) for at least two minutes. The cells will be spun down into a pellet, while the phage will remain in the supernatant. Save the supernatant in a separate microfuge tube. The phage will remain viable if stored at 4C. | ||
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Spin down | Spin down | ||
Host cells to log phase, after O/N | Host cells to log phase, after O/N | ||
Revision as of 21:53, 17 May 2017
1. Grow 3mL of cells with phage plasmid in liquid media overnight. These cells will produce M13 phage which can then be isolated. For example, grow J100265 + J100270 S1030 cells in 50ug/mLCarb + 50ug/mL Kan + 10ug/mL Tet for SPT7-spec phage.
2. Centrifuge 1.5mL of cells at full speed (~15,500 RPM) for at least two minutes. The cells will be spun down into a pellet, while the phage will remain in the supernatant. Save the supernatant in a separate microfuge tube. The phage will remain viable if stored at 4C.
Spin down Host cells to log phase, after O/N
