M13 Bacteriophage Production Protocol
From GcatWiki
1. Grow 3mL of cells with phage plasmid in liquid media overnight. These cells will produce M13 phage which can then be isolated. For example, grow J100265 + J100270 S1030 cells in 50ug/mLCarb + 50ug/mL Kan + 10ug/mL Tet for SPT7-spec phage.
2. Centrifuge 1.5mL of cells at full speed (~15,500 RPM) for at least two minutes. The cells will be spun down into a pellet, while the phage will remain in the supernatant. Save the supernatant in a separate microfuge tube. The phage will remain viable if stored at 4C.
Spin down Host cells to log phase, after O/N
