Difference between revisions of "Promoter Strength Metric"
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u=growth rate | u=growth rate | ||
| − | m=maturation constant for oxygen-dependent fluorophore activation of GFP | + | m=maturation constant for oxygen-dependent fluorophore activation of GFP<br> |
| − | D=first-order rate constant for protease-mediated degredation | + | D=first-order rate constant for protease-mediated degredation<br> |
| − | fss=exponential-phase steady state concentration of GFP | + | fss=exponential-phase steady state concentration of GFP<br> |
| − | P=rate of promoter driven production of GFP | + | P=rate of promoter driven production of GFP<br> |
Main Idea: | Main Idea: | ||
| − | Flouresence/absorbance per hour is measured by the rate of production, the amount of GFP in the cell, and the time GFP stays in the cell. | + | Flouresence/absorbance per hour is measured by the rate of production, the amount of GFP in the cell, and the time GFP stays in the cell. The equation is one of the measures used to characterize the mutant promoters' strength. |
Latest revision as of 02:21, 22 September 2007
P=Fss(u) (1+u/m)+ fssD((2u/m)+(D/m)+1)
u=growth rate
m=maturation constant for oxygen-dependent fluorophore activation of GFP
D=first-order rate constant for protease-mediated degredation
fss=exponential-phase steady state concentration of GFP
P=rate of promoter driven production of GFP
Main Idea:
Flouresence/absorbance per hour is measured by the rate of production, the amount of GFP in the cell, and the time GFP stays in the cell. The equation is one of the measures used to characterize the mutant promoters' strength.
