Difference between revisions of "Promoter Strength Metric"
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| Line 2: | Line 2: | ||
u=growth rate | u=growth rate | ||
| − | m=maturation constant for oxygen-dependent fluorophore activation of GFP | + | m=maturation constant for oxygen-dependent fluorophore activation of GFP<br> |
| − | D=first-order rate constant for protease-mediated degredation | + | D=first-order rate constant for protease-mediated degredation<br> |
| − | fss=exponential-phase steady state concentration of GFP | + | fss=exponential-phase steady state concentration of GFP<br> |
| − | P=rate of promoter driven production of GFP | + | P=rate of promoter driven production of GFP<br> |
Main Idea: | Main Idea: | ||
Flouresence/absorbance per hour is measured by the rate of production, the amount of GFP in the cell, and the time GFP stays in the cell. | Flouresence/absorbance per hour is measured by the rate of production, the amount of GFP in the cell, and the time GFP stays in the cell. | ||
Revision as of 02:21, 22 September 2007
P=Fss(u) (1+u/m)+ fssD((2u/m)+(D/m)+1)
u=growth rate
m=maturation constant for oxygen-dependent fluorophore activation of GFP
D=first-order rate constant for protease-mediated degredation
fss=exponential-phase steady state concentration of GFP
P=rate of promoter driven production of GFP
Main Idea:
Flouresence/absorbance per hour is measured by the rate of production, the amount of GFP in the cell, and the time GFP stays in the cell.
