Promoter Strength Metric

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P=Fss(u) (1+u/m)+ fssD((2u/m)+(D/m)+1)

u=growth rate m=maturation constant for oxygen-dependent fluorophore activation of GFP
D=first-order rate constant for protease-mediated degredation
fss=exponential-phase steady state concentration of GFP
P=rate of promoter driven production of GFP

Main Idea:

Flouresence/absorbance per hour is measured by the rate of production, the amount of GFP in the cell, and the time GFP stays in the cell.