Difference between revisions of "Tuning genetic control through promoter engineering"
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[http://www.bio.umass.edu/mcbfacs/intro.htm Helpful Flow Cytometry Link] | [http://www.bio.umass.edu/mcbfacs/intro.htm Helpful Flow Cytometry Link] | ||
| − | '''RT-PCR''' | + | '''RT-PCR''' <br> |
| − | In molecular biology, reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique for amplifying a defined piece of a ribonucleic acid (RNA) molecule. The RNA strand is first reverse transcribed into its DNA complement or complementary DNA, followed by amplification of the resulting DNA using polymerase chain reaction. | + | In molecular biology, reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique for amplifying a defined piece of a ribonucleic acid (RNA) molecule. The RNA strand is first reverse transcribed into its DNA complement or complementary DNA, followed by amplification of the resulting DNA using polymerase chain reaction. (from Wikipedia) |
[http://www.bio.davidson.edu/Courses/immunology/Flash/RT_PCR.html Davidson RT-PCR Animation] | [http://www.bio.davidson.edu/Courses/immunology/Flash/RT_PCR.html Davidson RT-PCR Animation] | ||
Revision as of 01:15, 22 September 2007
Methods
Flow Cytometry
Flow Cytometry is a technique for counting, examining, and sorting microscopic particles suspended in a stream of fluid. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection apparatus. (from Wikipedia)
RT-PCR
In molecular biology, reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique for amplifying a defined piece of a ribonucleic acid (RNA) molecule. The RNA strand is first reverse transcribed into its DNA complement or complementary DNA, followed by amplification of the resulting DNA using polymerase chain reaction. (from Wikipedia)
