Tuning genetic control through promoter engineering
Methods
Flow Cytometry
Flow Cytometry is a technique for counting, examining, and sorting microscopic particles suspended in a stream of fluid. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection apparatus. (from Wikipedia)
RT-PCR
In molecular biology, reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique for amplifying a defined piece of a ribonucleic acid (RNA) molecule. The RNA strand is first reverse transcribed into its DNA complement or complementary DNA, followed by amplification of the resulting DNA using polymerase chain reaction. (from Wikipedia)
Application of Promoter Library
Growth Yield Pathway
Phosphoenolpyruvate carboxylase on growth yield
Product Formation Pathway
Deoxy-xylulose P synthase on lycopene production
