XOR gate design involving Omp system

XOR gate design and concept

If all the components work as expected, the above construct will exhibit XOR gate properties. This design relies on a couple of assumptions:

1. When both inputs A (high osmolarity) and B (30C6), the activities of the two promoters should negate each other and no output should be produced.

2. When only one input is present (either high osmolarity or 30C6), the activity of the induced promoter should be able to reach the GFP gene despite the physical separation (the uninduced promoter) between the two components.

Testing the pOmpC promoter

Characterizing pOmpC

Is there any backwards activity?