PCR Primer Design

This webpage will add BioBrick ends to the input DNA sequence and design PCR primers (including four extra bases GCAT on each end for digestion purposes).

Before you submit your sequence, you need to know whether your part begins with an ATG or not. This web page assumes the part does NOT begin with a start codon (ATG).
See this web page for a full explanation, focus on the BioBrick prefix.

Please enter your DNA sequence (plain text or FASTA format):

Primer Size


About this Website

*This webpage, and the program used, was designed by James Barron, Madeline Parra, and Max Win in support of the Davidson College/Missouri Western iGEM 2008 project.

*Want to learn more about Davidson College's Biology Department? Click here.

*Want to learn more about Davidson College's Mathematics Department? Click here.

*What is iGEM??? Find out here.