Optimal Percent Agarose Concentration

A program for selecting the optimum percent agarose gel concentration for a given base pair length of dsDNA.


In these experiments, a 1kb ladder was run on different percent agarose gel concentrations (0.4, 0.6, 1.0, 1.4, 1.8, 2.1, 2.5 and 3.0%). All gels were produced using 60 mL of 0.5X TBE buffer solution, 0.2 μg/μLethidium bromide and variable amount of agarose. All gels were run in a 0.5X TBE buffer for 60 minutes at 100V and 400A. The gels were photographed next to a fluorescent ruler to use as an internal size standard. The mobility of each base pair length was measured in ImageJ. The data were analyzed in Excel and Mathematica.


About This Website


*Methods for determining the optimum percent agarose gel concentration

*Want to learn how to read a 1kb ladder? Click here.

*This webpage and the program used was designed by Kelly Davis and Max Win, in support of the Davidson College/Missouri Western iGEM 2008 project.

*Want to learn more about Davidson College's Biology Department? Click here.

*Want to learn more about Davidson College's Mathematics Department? Click here.

*What is iGEM??? Find out here.

Send comments, questions, and suggestions to: Dr. A. Malcolm Campbell or Dr. Laurie J. Heyer.

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