A program for selecting the optimum percent agarose gel concentration for a given base pair length of dsDNA.
Methods
In these experiments, a 1kb ladder was run on different percent agarose gel concentrations (0.4, 0.6, 1.0, 1.4, 1.8, 2.1, 2.5 and 3.0%). All gels were produced using 60 mL of 0.5X TBE buffer solution, 0.2 μg/μLethidium bromide and variable amount of agarose. All gels were run in a 0.5X TBE buffer for 60 minutes at 100V and 400A. The gels were photographed next to a fluorescent ruler to use as an internal size standard. The mobility of each base pair length was measured in ImageJ. The data were analyzed in Excel and Mathematica.
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