Difference between revisions of "J-GGA Scaffold 2.0 Sequence"
From GcatWiki
(Created page with "Scaffold 2.0 Top (5’to 3’) AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGGTCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA Scaffold 2.0 Bo...") |
|||
| Line 20: | Line 20: | ||
GCGCCGGCGAAGATCTCGGCATATGTGTCTCCTGGTGCAACCCAGAACTTGGTCCTCGCCTCACGATTACTGACACGCTAATCCGGATTGCTTGTCTGTCGATGATCATCGCCGGCG | GCGCCGGCGAAGATCTCGGCATATGTGTCTCCTGGTGCAACCCAGAACTTGGTCCTCGCCTCACGATTACTGACACGCTAATCCGGATTGCTTGTCTGTCGATGATCATCGCCGGCG | ||
| + | |||
| + | |||
| + | Scaffold 2.0 Ordering | ||
| + | |||
| + | Scaffold2.0_Top1; AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGG | ||
| + | |||
| + | Scaffold2.0_Top2; TCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA | ||
| + | |||
| + | Scaffold2.0_Bottom1; GCGGCCGCTACTAGTAGCTGTCTGTTCGTTAGGCCTAATCGCAC | ||
| + | |||
| + | Scaffold2.0_Bottom2; AGTCATTAGCACTCCGCTCCTGGTTCAAGACCCAACGTGGTCCTCTGTGTATACGGCTCTAGAAGCGGCCGCG | ||
Latest revision as of 17:07, 30 July 2013
Scaffold 2.0 Top
(5’to 3’)
AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGGTCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA
Scaffold 2.0 Bottom
(5’to 3’)
GCGGCCGCTACTAGTAGCTGTCTGTTCGTTAGGCCTAATCGCACAGTCATTAGCACTCCGCTCCTGGTTCAAGACCCAACGTGGTCCTCTGTGTATACGGCTCTAGAAGCGGCCGCG
Annealed Scaffold Top and Bottom Oligos
Prefix Junction A Junction B Junction C Junction D suffix
AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGGTCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA
GCGCCGGCGAAGATCTCGGCATATGTGTCTCCTGGTGCAACCCAGAACTTGGTCCTCGCCTCACGATTACTGACACGCTAATCCGGATTGCTTGTCTGTCGATGATCATCGCCGGCG
Scaffold 2.0 Ordering
Scaffold2.0_Top1; AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGG
Scaffold2.0_Top2; TCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA
Scaffold2.0_Bottom1; GCGGCCGCTACTAGTAGCTGTCTGTTCGTTAGGCCTAATCGCAC
Scaffold2.0_Bottom2; AGTCATTAGCACTCCGCTCCTGGTTCAAGACCCAACGTGGTCCTCTGTGTATACGGCTCTAGAAGCGGCCGCG
