J-GGA Scaffold 2.0 Sequence

Scaffold 2.0 Top

(5’to 3’)

AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGGTCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA

Scaffold 2.0 Bottom

(5’to 3’)

GCGGCCGCTACTAGTAGCTGTCTGTTCGTTAGGCCTAATCGCACAGTCATTAGCACTCCGCTCCTGGTTCAAGACCCAACGTGGTCCTCTGTGTATACGGCTCTAGAAGCGGCCGCG

Annealed Scaffold Top and Bottom Oligos

  Prefix          Junction A               Junction B               Junction C              Junction D         suffix

AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGGTCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA

    GCGCCGGCGAAGATCTCGGCATATGTGTCTCCTGGTGCAACCCAGAACTTGGTCCTCGCCTCACGATTACTGACACGCTAATCCGGATTGCTTGTCTGTCGATGATCATCGCCGGCG

Scaffold 2.0 Ordering

Scaffold2.0_Top1; AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGG

Scaffold2.0_Top2; TCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA

Scaffold2.0_Bottom1; GCGGCCGCTACTAGTAGCTGTCTGTTCGTTAGGCCTAATCGCAC

Scaffold2.0_Bottom2; AGTCATTAGCACTCCGCTCCTGGTTCAAGACCCAACGTGGTCCTCTGTGTATACGGCTCTAGAAGCGGCCGCG