Difference between revisions of "Primer Design for the RFP"
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Latest revision as of 17:11, 30 July 2013
RFP for scaffold 2.0
Forward primer will bind to 5’ end of RFP
JGGA2.0_RFP_for; GCATGGTCTCTGCGAGCTTCCTCCGAAGACGTTATC
Reverse primer will to 3’ end of RFP
JGGA2.0_RFP_rev; GCATGGTCTCAAGGCTTATTAAGCACCGGTGGAG
Primers for RFP Insertion between Junctions C and D
Oligos to be Ordered
JGGA2.0_RFP_for; GCATGGTCTCTGCGAGCTTCCTCCGAAGACGTTATC
JGGA2.0_RFP_rev; GCATGGTCTCAAGGCTTATTAAGCACCGGTGGAG
>BBa_E1010 Part-only sequence (706 bp)
Atg gct tcc tcc gaa gac gtt atc aaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtg
aaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagta
cggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaa
gacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtc
cggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaact
gaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggac
atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca
ctccaccggtgcttaataa
