Primer Design for the RFP

RFP for scaffold 2.0

Forward primer will bind to 5’ end of RFP

JGGA2.0_RFP_for; GCATGGTCTCTGCGAGCTTCCTCCGAAGACGTTATC

Reverse primer will to 3’ end of RFP

JGGA2.0_RFP_rev; GCATGGTCTCAAGGCTTATTAAGCACCGGTGGAG

Primers for RFP Insertion between Junctions C and D

Oligos to be Ordered

JGGA2.0_RFP_for; GCATGGTCTCTGCGAGCTTCCTCCGAAGACGTTATC

JGGA2.0_RFP_rev; GCATGGTCTCAAGGCTTATTAAGCACCGGTGGAG

>BBa_E1010 Part-only sequence (706 bp)

Atg gct tcc tcc gaa gac gtt atc aaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtg

aaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagta

cggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaa

gacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtc

cggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaact

gaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggac

atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca

ctccaccggtgcttaataa