Difference between revisions of "LongAmp PCR"
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Revision as of 18:32, 1 July 2014
Preparation
1. 0.6uL 10mM dNTPs
2. 1uL 1ng template
3. 1uL 10uM forward primer
4. 1uL 10uM reverse primer
5. 1uL LongAmp Taq Polymerase
6. 4uL LongAmp Buffer
7. 11.4uL dH20
Programed PCR Protocol
Stage 1:
94ºC 5 minutes
Stage 2:
30 cycles
94ºC 30 seconds
60ºC-->45ºC 30 seconds
60ºC 10 minutes
Stage 3:
72ºC 10 minutes
