LongAmp PCR

Preparation

1. 0.6uL 10mM dNTPs

2. 1uL 1ng template

3. 1uL 10uM forward primer

4. 1uL 10uM reverse primer

5. 1uL LongAmp Taq Polymerase

6. 4uL 5X LongAmp Buffer

7. 11.4uL dH20

Programed PCR Protocol

Stage 1:

94ºC 5 minutes

Stage 2:

30 cycles

94ºC 30 seconds

55ºC 30 seconds (Or a different annealing temperature)

65ºC 10 minutes (Or 1 minute per 1000bp)

Stage 3:

65ºC 10 minutes