Difference between revisions of "Common Restriction Sites"
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(Created page with "3’ Overhang: 2 Sticky Ends- * AsiSI * * PacI * PvuI-HF * * SacII * 4 Sticky Ends- • AatII * • BmtI/BmtI-HF • FseI * • KpnI-HF • NsiI-HF • PstI-HF • SacI-HF *...") |
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| − | 3’ Overhang: | + | '''These restriction sites are all optimal at 37 degrees Celsius and are organized by the kind of overhang they create. *denotes it is affected by methylation (CpG, dcm, or dam)''' |
| + | |||
| + | ---- | ||
| + | |||
| + | '''3’ Overhang:''' | ||
| + | |||
2 Sticky Ends- | 2 Sticky Ends- | ||
* AsiSI * | * AsiSI * | ||
| Line 6: | Line 11: | ||
* SacII * | * SacII * | ||
4 Sticky Ends- | 4 Sticky Ends- | ||
| − | + | * AatII * | |
| − | + | * BmtI/BmtI-HF | |
| − | + | * FseI * | |
| − | + | * KpnI-HF | |
| − | + | * NsiI-HF | |
| − | + | * PstI-HF | |
| − | + | * SacI-HF * | |
| − | + | * SbfI/SbfI-HF | |
| − | + | * SphI-HF | |
| + | |||
| + | '''5’ Overhang:''' | ||
| + | |||
| + | 2 Sticky Ends- | ||
| + | * AcII: * | ||
| + | * ClaI * | ||
| + | * NarI * | ||
| + | * NdeI | ||
| + | 4 Sticky Ends- | ||
| + | * AflII | ||
| + | * AgeI-HF | ||
| + | * ApaLI * | ||
| + | * AscI * | ||
| + | * AvrII | ||
| + | * BamHI-HF | ||
| + | * BclI-HF * | ||
| + | * BsaI-HF V2 * | ||
| + | * BspHI * | ||
| + | * BsrGI-HF | ||
| + | * EagI-HF * | ||
| + | * EcoRI-HF * | ||
| + | * HindIII-HF | ||
| + | * KasI * | ||
| + | * MfeI/MfeI-HF | ||
| + | * MluI-HF * | ||
| + | * NcoI-HF | ||
| + | * NgoMIV * | ||
| + | * NheI-HF 4SE * | ||
| + | * NotI-HF * | ||
| + | * PspOMI * | ||
| + | * SalI-HF * | ||
| + | * SpeI/SpeI-HF | ||
| + | * XbaI * | ||
| + | * XhoI * | ||
| + | * XmaI * | ||
| + | |||
| + | '''Blunt cutters:''' | ||
| + | * AfeI * | ||
| + | * BsrBI* | ||
| + | * DraI | ||
| + | * EcoRV * | ||
| + | * FspI * | ||
| + | * HpaI * | ||
| + | * MscI * | ||
| + | * NaeI * | ||
| + | * NruI-HF * | ||
| + | * PmeI * | ||
| + | * PmlI * | ||
| + | * PvuII-HF | ||
| + | * ScaI-HF | ||
| + | * SfoI * | ||
| + | * SnaBI * | ||
| + | * SspI-HF | ||
| + | * StuI * | ||
Latest revision as of 23:59, 27 September 2018
These restriction sites are all optimal at 37 degrees Celsius and are organized by the kind of overhang they create. *denotes it is affected by methylation (CpG, dcm, or dam)
3’ Overhang:
2 Sticky Ends-
- AsiSI *
- PacI
- PvuI-HF *
- SacII *
4 Sticky Ends-
- AatII *
- BmtI/BmtI-HF
- FseI *
- KpnI-HF
- NsiI-HF
- PstI-HF
- SacI-HF *
- SbfI/SbfI-HF
- SphI-HF
5’ Overhang:
2 Sticky Ends-
- AcII: *
- ClaI *
- NarI *
- NdeI
4 Sticky Ends-
- AflII
- AgeI-HF
- ApaLI *
- AscI *
- AvrII
- BamHI-HF
- BclI-HF *
- BsaI-HF V2 *
- BspHI *
- BsrGI-HF
- EagI-HF *
- EcoRI-HF *
- HindIII-HF
- KasI *
- MfeI/MfeI-HF
- MluI-HF *
- NcoI-HF
- NgoMIV *
- NheI-HF 4SE *
- NotI-HF *
- PspOMI *
- SalI-HF *
- SpeI/SpeI-HF
- XbaI *
- XhoI *
- XmaI *
Blunt cutters:
- AfeI *
- BsrBI*
- DraI
- EcoRV *
- FspI *
- HpaI *
- MscI *
- NaeI *
- NruI-HF *
- PmeI *
- PmlI *
- PvuII-HF
- ScaI-HF
- SfoI *
- SnaBI *
- SspI-HF
- StuI *
