Common Restriction Sites

These restriction sites are all optimal at 37 degrees Celsius and are organized by the kind of overhang they create. *denotes it is affected by methylation (CpG, dcm, or dam)

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3’ Overhang:

2 Sticky Ends-

• AsiSI *
• PacI
• PvuI-HF *
• SacII *

4 Sticky Ends-

• AatII *
• BmtI/BmtI-HF
• FseI *
• KpnI-HF
• NsiI-HF
• PstI-HF
• SacI-HF *
• SbfI/SbfI-HF
• SphI-HF

5’ Overhang:

2 Sticky Ends-

• AcII: *
• ClaI *
• NarI *
• NdeI

4 Sticky Ends-

• AflII
• AgeI-HF
• ApaLI *
• AscI *
• AvrII
• BamHI-HF
• BclI-HF *
• BsaI-HF V2 *
• BspHI *
• BsrGI-HF
• EagI-HF *
• EcoRI-HF *
• HindIII-HF
• KasI *
• MfeI/MfeI-HF
• MluI-HF *
• NcoI-HF
• NgoMIV *
• NheI-HF 4SE *
• NotI-HF *
• PspOMI *
• SalI-HF *
• SpeI/SpeI-HF
• XbaI *
• XhoI *
• XmaI *

Blunt cutters:

• AfeI *
• BsrBI*
• DraI
• EcoRV *
• FspI *
• HpaI *
• MscI *
• NaeI *
• NruI-HF *
• PmeI *
• PmlI *
• PvuII-HF
• ScaI-HF
• SfoI *
• SnaBI *
• SspI-HF
• StuI *