Difference between revisions of "Test pLsrR/cI"
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pLsrA/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off. | pLsrA/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off. | ||
| − | 1)Test LsrA repression. | + | '''1)Test LsrA repression.''' |
| − | Some promoter→RBS+LsrR+ | + | Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP does not glow |
| + | |||
| + | . B0034+K091001+B0015+K091106→E0240 | ||
add AI-2 | add AI-2 | ||
| − | Some promoter→RBS+LsrR+ | + | Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP glows |
| + | |||
| + | . B0034+K091001+B0015+K091106→E0240 | ||
| − | 2)Test cI repression. | + | '''2)Test cI repression.''' |
| − | Some promoter→RBS+cI+ | + | Some promoter→RBS+cI+TT+pLsrA/cI→GFP does not glow |
| + | |||
| + | . B0034+C0051+B0015+K091106→E0240 | ||
add cI | add cI | ||
| − | Some promoter→RBS+cI+ | + | Some promoter→RBS+cI+TT+pLsrA/cI→GFP does not glow |
| + | |||
| + | . B0034+C0051+B0015+K091106→E0240 | ||
| − | 3)Test LsrA and cI repression. | + | '''3)Test LsrA and cI repression.''' |
| − | Some | + | Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow |
| + | |||
| + | . B0034+K091001+C0051+B0015+K091106→E0240 | ||
add AI-2 | add AI-2 | ||
| − | Some | + | Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow |
| + | |||
| + | . B0034+K091001+C0051+B0015+K091106→E0240 | ||
| − | 4)Control. | + | |
| + | '''4)Control.''' | ||
pLsrR/cI→GFP glows | pLsrR/cI→GFP glows | ||
| + | |||
| + | K091106→E0240 | ||
| + | |||
{| border="1" cellpadding="2" | {| border="1" cellpadding="2" | ||
| Line 36: | Line 52: | ||
!width="30"|AI-2 + GFP | !width="30"|AI-2 + GFP | ||
!width="30"|AI-2 - GFP | !width="30"|AI-2 - GFP | ||
| − | |||
| − | |||
|- | |- | ||
| 1 || 0 || 1 || 0 | | 1 || 0 || 1 || 0 | ||
| Line 44: | Line 58: | ||
|- | |- | ||
| 1 || 1 || 0 || 0 | | 1 || 1 || 0 || 0 | ||
| + | |- | ||
| + | | 0 || 0 || 1 || 1 | ||
|} | |} | ||
Latest revision as of 14:05, 1 July 2008
pLsrA/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.
1)Test LsrA repression.
Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP does not glow
. B0034+K091001+B0015+K091106→E0240
add AI-2
Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP glows
. B0034+K091001+B0015+K091106→E0240
2)Test cI repression.
Some promoter→RBS+cI+TT+pLsrA/cI→GFP does not glow
. B0034+C0051+B0015+K091106→E0240
add cI
Some promoter→RBS+cI+TT+pLsrA/cI→GFP does not glow
. B0034+C0051+B0015+K091106→E0240
3)Test LsrA and cI repression.
Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow
. B0034+K091001+C0051+B0015+K091106→E0240
add AI-2
Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow
. B0034+K091001+C0051+B0015+K091106→E0240
4)Control.
pLsrR/cI→GFP glows
K091106→E0240
| LsrA | cI | AI-2 + GFP | AI-2 - GFP |
|---|---|---|---|
| 1 | 0 | 1 | 0 |
| 0 | 1 | 0 | 0 |
| 1 | 1 | 0 | 0 |
| 0 | 0 | 1 | 1 |
