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| − | This is a rough idea of how to test the four hybrid promoters for the XOR gate. | + | This are our ideas of how to test the four hybrid promoters for the XOR gate. |
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| − | '''[[Test pLuxR/cI]]
| + | [[Test pLuxR/cI]] |
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| − | '''Test pMnt/Lac'''
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| − | [[pMnt/Lac]] | + | [[Test pMnt/Lac]] |
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| − | pMnt/Lac is constitutively on but is repressed by the LacI protein. The promoter is also repressed by the Mnt protein. The addition of IPTG should repress the LacI repressor and glow in the absence of the Mnt protein.
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| − | Some promoter→LacI+t+t+pLac/Mnt→GFP does not glow
| + | [[Test pLsrA/cI]] |
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| − | Some promoter→Mnt+t+t+pLac/Mnt→GFP does not glow
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| − |
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| − | add IPTG
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| − | Some promoter→LacI+t+t+pLac/Mnt→GFP glows
| + | [[Test pLasR/cI]] |
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| − | {| border="1" cellpadding="2"
| + | [[Test Mnt/Tet promoter]] |
| − | !width="30"|LacI
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| − | !width="30"|Mnt
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| − | !width="30"|IPTG + GFP
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| − | !width="30"|IPTG - GFP
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| − | |-
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| − | | 0 || 0 || 1 || 1
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| − | |-
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| − | | 0 || 1 || 0 || 0
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| − | |-
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| − | | 1 || 0 || 1 || 0
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| − | |-
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| − | | 1 || 1 || 0 || 0
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| − | |}
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| − | [[Test pLsrR/cI]] | |
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| − | [[Test pLasR/cI]]
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| − | | |
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| − | [[Test Mnt/Tet promoter]]
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| − | The pMnt/TetR promoter is constitutively on and is repressed by the TetR protein. The addition of aTc should repress the TetR causing it to glow. The addition of the Mnt protein should repress the promoter causing it not glow.
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| | + | '''Overlapping Parts And intermediates''' |
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| | {| border="1" cellpadding="2" | | {| border="1" cellpadding="2" |
| − | !width="30"|TetR | + | !width="30"|LuxR/cI |
| − | !width="30"|Mnt | + | !width="30"|Lac/Mnt |
| − | !width="30"|aTc + GFP | + | !width="30"|LsrR/cI |
| − | !width="30"|aTc - GFP | + | !width="30"|LasR/cI |
| | + | !width="30"|Mnt/Tet |
| | |- | | |- |
| − | | 0 || 0 || 1 || 1 | + | | RBS+cI(+TT) || || RBS+cI(+TT) || || |
| | |- | | |- |
| − | | 0 || 1 || 0 || 0 | + | | || RBS+Mnt(+TT) || || RBS+Mnt(+TT) || |
| | |- | | |- |
| − | | 1 || 0 || 1 || 0 | + | | || || || || |
| | |- | | |- |
| − | | 1 || 1 || 0 || 0 | + | | || || || || |
| | |} | | |} |
| − |
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| − |
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| − | Test tet repression
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| − |
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| − |
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| − | Some promoter (pbad)→RBS+TetR+t+t+pMnt/Tet→RBS+GFP does not glows
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| − |
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| − | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13453 I13453]+RBS+[http://partsregistry.org/Part:BBa_C0040 C0040]+TT+[http://partsregistry.org/Part:BBa_K091105 K091105]+[http://partsregistry.org/Part:BBa_E0240 E0240]
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| − |
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| − |
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| − | add aTc
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| − |
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| − | Some promoter→TetR+t+t+pMnt/Tet→GFP glows
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| − |
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| − | I13453+RBS+C0040+TT+K091105+E0240
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| − |
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| − |
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| − | Test Mnt repression:
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| − |
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| − | Some promoter→Mnt+t+t+pMnt/Tet→GFP glows
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| − |
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| − | I13453+RBS+[http://partsregistry.org/Part:BBa_C0072 C0072]+TT+K091105+E0240
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| − |
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| − |
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| − | Test Tet and Mnt repression:
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| − |
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| − | Some promoter→TetR+Mnt+t+t+pMnt/Tet→GFP does not glows
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| − |
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| − | I13453+RBS+C0040+C0072+TT+K091105+E0240
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| − |
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| − | add aTc does not glow
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