Test pLasR/cI
From GcatWiki
pLasR/cI is constitutively off and is repressed by cI. The addition of p-AI-1, activates LasR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.
| LasR | cI | p-AI-1 + GFP | p-AI-1 - GFP |
|---|---|---|---|
| 0 | 0 | 0 | 0 |
| 1 | 0 | 1 | 0 |
| 0 | 1 | 0 | 0 |
| 1 | 1 | 0 | 0 |
Test pLasR/cI:
pBAD→RBS+LasR+TT (from James DC)
I13453+B0034+C0079+B0015
plasR/cI→RBS+GFP+tt
K09110?+E0240 does not glow
add P-AI-1
pBAD→RBS+LasR+TT (from James DC)
I13453+B0034+C0079+B0015
plasR/cI→RBS+GFP+tt
K09110?+E0240 glows
Ligation plan for test sequences
Digest plasR/cI with S/P, E0240 with X/P; do ligation.
