Difference between revisions of "LsrA/cI"

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'''Goal''': pBAD+RBS+lsrR+RBS+cI+TT+pLsrA/cI+RBS+LuxI+RBS+Mnt+TT
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'''Goal''':  
  
−
I13453+B0034+K091001+B0034+C0051+B0015+K091106+B0034+C0061+B0034+C0072+B0015
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'''pBAD+RBS+lsrK+RBS+lsrR+TT'''
  
 +
'''pLsrA/cI+RBS+LuxI+RBS+Mnt+TT'''
 +
 +
'''I13453'''+'''B0034'''+'''K091002'''+'''B0034'''+'''K091001'''+'''B0015'''
 +
 +
'''K091106'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''
 +
 +
 +
'''Ligation Plan'''
 
{| border="1" cellpadding="2"
 
{| border="1" cellpadding="2"
 
!width="20"|
 
!width="20"|
 
!width="30"|Vector
 
!width="30"|Vector
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!width="70"|
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!width="110"|
 
!width="30"|
 
!width="30"|
 
!width="30"|
 
!width="30"|
 
!width="30"|
 
!width="30"|
 
!width="30"|Insert
 
!width="30"|Insert
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!width="70"|
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!width="110"|
 
!width="30"|
 
!width="30"|
 
!width="30"|
 
!width="30"|
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|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''
 
|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''
 
|-
 
|-
−
| 1|| I13453||pBAD  ||R/X;S/P  ||S/P  ||120 ||B0034 ||RBS ||R/X; S/P||X/P || 12|| ||
+
| 1|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091001||lsrR  ||R/X;S/P  ||X/P  ||954 ||K091138 ||972
 +
|-
 +
| 2|| B0034 ||RBS ||R/X; S/P||S/P || 12+2079||K091002||lsrK  ||R/X;S/P  ||X/P  ||1593 ||K091158 ||1611
 +
|-
 +
| 3||I13453||pBAD ||R/X;S/P ||S/P || 120+2079||TBD||RBS+lsrK ||R/X; S/P ||X/P  ||1611 ||K091159 || 1866
 +
|-
 +
| 4||B0015||TT||R/X; S/P ||R/X ||129+2079 ||K091138 ||RBS+lsrR ||R/X; S/P  ||R/S  || 972||K091139 ||1109
 +
|-
 +
| 5||K091139||RBS+lsrR+TT||R/X; S/P ||R/X ||1109+2079 ||TBD ||pBAD+RBA+lsrK  ||R/X; S/P  ||R/S  ||1866 ||TBD ||
 +
|-
 +
| 6|| K091106 ||pLsrA/cI ||R/X; S/P ||S/P || 141+2079||C0261||RBS+luxI  ||R/X; S/P  ||X/P  ||661 ||K091140||810
 +
|-
 +
| 7||B0015 ||TT ||R/X; S/P ||R/X ||129+2079 ||I13025 ||RBS+Mnt  ||R/X;S/P  ||R/S  ||331 ||K091141 ||468
 +
|-
 +
| 8||K091141||RBS+Mnt+TT ||R/X; S/P ||R/X || 468+2079||K091140 ||pLsrA/cI+RBS+luxI  ||R/X; S/P ||R/S  ||810 || K091142||1286
 +
|-
 +
|}
 +
 
 +
'''Parts'''
 +
{| border="1" cellpadding="2"
 +
!width="40"|BBa_
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!width="110"|Description
 +
!width="60"|Source
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!width="100"|location('07)
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!width="35"|Vector
 +
!width="40"|Responsible
 +
|-
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|[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 ||
 +
|-
 +
|[http://partsregistry.org/Part:BBa_B0034 B0034] ||RBS ||registry 07 ||Plt.1-3O ||pSB1A2 ||  
 
|-
 
|-
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| 2||K091106||lsrR ||R/X; S/P  ||R/X  ||954 || ||pBAD+RBS ||R/X;S/P ||R/S || || ||
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|[http://partsregistry.org/Part:BBa_I13453 I13453] ||pBAD ||registry 07 ||Plt.2-13D; Plt.3-3I ||pSB1A3 ||  
 
|-
 
|-
−
| 3|| ||pBAD+RBS+lsrR  ||R/X; S/P  ||S/P  || ||P0455 ||RBS+cI+TT ||R/X; S/P ||X/P ||896 || ||
+
|[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07  ||Plt.1-24A ||pSB1A2 ||  
 
|-
 
|-
−
| || ||  || ||  || || || || || || || ||
+
|[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 ||  
 
|-
 
|-
−
| || ||  || ||  || || || || || || || ||
+
|[http://partsregistry.org/Part:BBa_K091001 K091001] ||lsrR ||MW ||N/A||N/A ||  
 
|-
 
|-
−
| || ||  || ||  || || || || || || || ||
+
|[http://partsregistry.org/Part:BBa_K091002 K091002] ||lsrK ||MW ||N/A||N/A ||  
 
|-
 
|-
−
| || ||  || ||  || || || || || || || ||
+
|[http://partsregistry.org/Part:BBa_K091106 K091106] ||plsrA/cI ||MW ||N/A ||N/A ||  
 
|-
 
|-
 
|}
 
|}

Latest revision as of 15:27, 28 July 2008

Goal:

pBAD+RBS+lsrK+RBS+lsrR+TT

pLsrA/cI+RBS+LuxI+RBS+Mnt+TT

I13453+B0034+K091002+B0034+K091001+B0015

K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015


Ligation Plan

Vector Insert Product
Step BBa_ Description Pre-/suffix Enzymes Size BBa_ Description Pre-/suffix Enzymes Size BBa_ Size
1 B0034 RBS R/X; S/P S/P 12+2079 K091001 lsrR R/X;S/P X/P 954 K091138 972
2 B0034 RBS R/X; S/P S/P 12+2079 K091002 lsrK R/X;S/P X/P 1593 K091158 1611
3 I13453 pBAD R/X;S/P S/P 120+2079 TBD RBS+lsrK R/X; S/P X/P 1611 K091159 1866
4 B0015 TT R/X; S/P R/X 129+2079 K091138 RBS+lsrR R/X; S/P R/S 972 K091139 1109
5 K091139 RBS+lsrR+TT R/X; S/P R/X 1109+2079 TBD pBAD+RBA+lsrK R/X; S/P R/S 1866 TBD
6 K091106 pLsrA/cI R/X; S/P S/P 141+2079 C0261 RBS+luxI R/X; S/P X/P 661 K091140 810
7 B0015 TT R/X; S/P R/X 129+2079 I13025 RBS+Mnt R/X;S/P R/S 331 K091141 468
8 K091141 RBS+Mnt+TT R/X; S/P R/X 468+2079 K091140 pLsrA/cI+RBS+luxI R/X; S/P R/S 810 K091142 1286

Parts

BBa_ Description Source location('07) Vector Responsible
B0015 double terminator registry 07 Plt.1-1I;Plt.3-3O pSB1AK3
B0034 RBS registry 07 Plt.1-3O pSB1A2
I13453 pBAD registry 07 Plt.2-13D; Plt.3-3I pSB1A3
C0261 RBS+luxI registry 07 Plt.1-24A pSB1A2
I13025 RBS+Mnt registry 07 Plt.1-23J pSB1A2
K091001 lsrR MW N/A N/A
K091002 lsrK MW N/A N/A
K091106 plsrA/cI MW N/A N/A
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