LsrA/cI

Goal:

pBAD+RBS+lsrK+RBS+lsrR+TT

pLsrA/cI+RBS+LuxI+RBS+Mnt+TT

I13453+B0034+K091002+B0034+K091001+B0015

K091106+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015

Ligation Plan

	Vector					Insert					Product
Step	BBa_	Description	Pre-/suffix	Enzymes	Size	BBa_	Description	Pre-/suffix	Enzymes	Size	BBa_	Size
1	B0034	RBS	R/X; S/P	S/P	12+2079	K091001	lsrR	R/X;S/P	X/P	954	K091138	972
2	B0034	RBS	R/X; S/P	S/P	12+2079	K091002	lsrK	R/X;S/P	X/P	1593	K091158	1611
3	I13453	pBAD	R/X;S/P	S/P	120+2079	TBD	RBS+lsrK	R/X; S/P	X/P	1611	K091159	1866
4	B0015	TT	R/X; S/P	R/X	129+2079	K091138	RBS+lsrR	R/X; S/P	R/S	972	K091139	1109
5	K091139	RBS+lsrR+TT	R/X; S/P	R/X	1109+2079	TBD	pBAD+RBA+lsrK	R/X; S/P	R/S	1866	TBD
6	K091106	pLsrA/cI	R/X; S/P	S/P	141+2079	C0261	RBS+luxI	R/X; S/P	X/P	661	K091140	810
7	B0015	TT	R/X; S/P	R/X	129+2079	I13025	RBS+Mnt	R/X;S/P	R/S	331	K091141	468
8	K091141	RBS+Mnt+TT	R/X; S/P	R/X	468+2079	K091140	pLsrA/cI+RBS+luxI	R/X; S/P	R/S	810	K091142	1286

Parts

BBa_	Description	Source	location('07)	Vector	Responsible
B0015	double terminator	registry 07	Plt.1-1I;Plt.3-3O	pSB1AK3
B0034	RBS	registry 07	Plt.1-3O	pSB1A2
I13453	pBAD	registry 07	Plt.2-13D; Plt.3-3I	pSB1A3
C0261	RBS+luxI	registry 07	Plt.1-24A	pSB1A2
I13025	RBS+Mnt	registry 07	Plt.1-23J	pSB1A2
K091001	lsrR	MW	N/A	N/A
K091002	lsrK	MW	N/A	N/A
K091106	plsrA/cI	MW	N/A	N/A