Difference between revisions of "Test pLsrR/cI"
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| − | + | pLsrA/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off. | |
| − | + | '''1)Test LsrA repression.''' | |
| + | Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP does not glow | ||
| + | |||
| + | . B0034+K091001+B0015+K091106→E0240 | ||
add AI-2 | add AI-2 | ||
| − | Some | + | Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP glows |
| + | |||
| + | . B0034+K091001+B0015+K091106→E0240 | ||
| + | |||
| + | '''2)Test cI repression.''' | ||
| + | |||
| + | Some promoter→RBS+cI+TT+pLsrA/cI→GFP does not glow | ||
| + | |||
| + | . B0034+C0051+B0015+K091106→E0240 | ||
| + | |||
| + | add cI | ||
| + | |||
| + | Some promoter→RBS+cI+TT+pLsrA/cI→GFP does not glow | ||
| + | |||
| + | . B0034+C0051+B0015+K091106→E0240 | ||
| + | |||
| + | |||
| + | '''3)Test LsrA and cI repression.''' | ||
| + | |||
| + | Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow | ||
| + | |||
| + | . B0034+K091001+C0051+B0015+K091106→E0240 | ||
add AI-2 | add AI-2 | ||
| − | Some | + | Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow |
| + | |||
| + | . B0034+K091001+C0051+B0015+K091106→E0240 | ||
| + | |||
| + | |||
| + | '''4)Control.''' | ||
| + | |||
| + | pLsrR/cI→GFP glows | ||
| + | |||
| + | K091106→E0240 | ||
| + | |||
{| border="1" cellpadding="2" | {| border="1" cellpadding="2" | ||
| − | !width="30"| | + | !width="30"|LsrA |
!width="30"|cI | !width="30"|cI | ||
!width="30"|AI-2 + GFP | !width="30"|AI-2 + GFP | ||
!width="30"|AI-2 - GFP | !width="30"|AI-2 - GFP | ||
| − | |||
| − | |||
|- | |- | ||
| 1 || 0 || 1 || 0 | | 1 || 0 || 1 || 0 | ||
| Line 26: | Line 58: | ||
|- | |- | ||
| 1 || 1 || 0 || 0 | | 1 || 1 || 0 || 0 | ||
| + | |- | ||
| + | | 0 || 0 || 1 || 1 | ||
|} | |} | ||
Latest revision as of 14:05, 1 July 2008
pLsrA/cI is constitutively on and is repressed by LsrR. The addition of AI-2, which becomes phosphorylated by LsrK, represses LsrR causing the promoter to turn on. The addition of cI to should repress the promoter causing it to turn off.
1)Test LsrA repression.
Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP does not glow
. B0034+K091001+B0015+K091106→E0240
add AI-2
Some promoter→RBS+LsrR+TT+pLsrA/cI→GFP glows
. B0034+K091001+B0015+K091106→E0240
2)Test cI repression.
Some promoter→RBS+cI+TT+pLsrA/cI→GFP does not glow
. B0034+C0051+B0015+K091106→E0240
add cI
Some promoter→RBS+cI+TT+pLsrA/cI→GFP does not glow
. B0034+C0051+B0015+K091106→E0240
3)Test LsrA and cI repression.
Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow
. B0034+K091001+C0051+B0015+K091106→E0240
add AI-2
Some promoter→RBS+LsrR+cI+TT+pLsrR/cI→GFP does not glow
. B0034+K091001+C0051+B0015+K091106→E0240
4)Control.
pLsrR/cI→GFP glows
K091106→E0240
| LsrA | cI | AI-2 + GFP | AI-2 - GFP |
|---|---|---|---|
| 1 | 0 | 1 | 0 |
| 0 | 1 | 0 | 0 |
| 1 | 1 | 0 | 0 |
| 0 | 0 | 1 | 1 |
