Difference between revisions of "LasR/cI"
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| (6 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
| − | + | '''Goal:''' | |
| − | I13453+B0034+C0079+ | + | '''pBAD+RBS+lasR+TT'''(James DC) |
| + | |||
| + | '''S0388?'''(I13453+B0034+C0079+B0015) | ||
| + | |||
| + | '''pLasR/cI+RBS+LuxI+RBS+Mnt+TT''' | ||
| + | |||
| + | '''K091143'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015''' | ||
| + | |||
| + | '''Ligation Plan''' | ||
| + | {| border="1" cellpadding="2" | ||
| + | !width="20"| | ||
| + | !width="30"|Vector | ||
| + | !width="110"| | ||
| + | !width="30"| | ||
| + | !width="30"| | ||
| + | !width="30"| | ||
| + | !width="30"|Insert | ||
| + | !width="110"| | ||
| + | !width="30"| | ||
| + | !width="30"| | ||
| + | !width="30"| | ||
| + | !width="30"|Product | ||
| + | !width="30"| | ||
| + | |- | ||
| + | |'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | ||
| + | |- | ||
| + | | 1|| K091141||RBS+Mnt+TT ||R/X;S/P ||R/X ||468+2079 ||C0261||RBS+luxI ||R/X;S/P ||R/X ||661 ||K091154 ||1137 | ||
| + | |- | ||
| + | | 2||K091143||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||K091154||RBS+luxI+RBS+Mnt+TT ||R/X; S/P ||X/P ||1137 ||K091155 ||1309 | ||
| + | |- | ||
| + | |} | ||
| + | |||
| + | '''Parts''' | ||
| + | {| border="1" cellpadding="2" | ||
| + | !width="40"|BBa_ | ||
| + | !width="110"|Description | ||
| + | !width="60"|Source | ||
| + | !width="100"|location('07) | ||
| + | !width="35"|Vector | ||
| + | !width="40"|Responsible | ||
| + | |- | ||
| + | |[http://partsregistry.org/Part:BBa_B0015 B0015]||double terminator ||registry 07 ||Plt.1-1I;Plt.3-3O ||pSB1AK3 || | ||
| + | |- | ||
| + | |[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07 ||Plt.1-23J ||pSB1A2 || | ||
| + | |- | ||
| + | |[http://partsregistry.org/Part:BBa_K091143 K091143] ||plasR/cI ||MW ||N/A ||pSB1A2 || | ||
| + | |- | ||
| + | |[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07 ||Plt.1-24A ||pSB1A2 || | ||
| + | |- | ||
| + | |} | ||
Latest revision as of 18:24, 22 July 2008
Goal:
pBAD+RBS+lasR+TT(James DC)
S0388?(I13453+B0034+C0079+B0015)
pLasR/cI+RBS+LuxI+RBS+Mnt+TT
K091143+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015
Ligation Plan
| Vector | Insert | Product | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size |
| 1 | K091141 | RBS+Mnt+TT | R/X;S/P | R/X | 468+2079 | C0261 | RBS+luxI | R/X;S/P | R/X | 661 | K091154 | 1137 |
| 2 | K091143 | plasR/cI | R/X;S/P | S/P | 164+2079 | K091154 | RBS+luxI+RBS+Mnt+TT | R/X; S/P | X/P | 1137 | K091155 | 1309 |
Parts
| BBa_ | Description | Source | location('07) | Vector | Responsible |
|---|---|---|---|---|---|
| B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 | |
| I13025 | RBS+Mnt | registry 07 | Plt.1-23J | pSB1A2 | |
| K091143 | plasR/cI | MW | N/A | pSB1A2 | |
| C0261 | RBS+luxI | registry 07 | Plt.1-24A | pSB1A2 |
