LasR/cI

Goal:

pBAD+RBS+lasR+TT(James DC)

S0388?(I13453+B0034+C0079+B0015)

pLasR/cI+RBS+LuxI+RBS+Mnt+TT

K091143+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015

Ligation Plan

	Vector					Insert					Product
Step	BBa_	Description	Pre-/suffix	Enzymes	Size	BBa_	Description	Pre-/suffix	Enzymes	Size	BBa_	Size
1	K091141	RBS+Mnt+TT	R/X;S/P	R/X	468+2079	C0261	RBS+luxI	R/X;S/P	R/X	661	K091154	1137
2	K091143	plasR/cI	R/X;S/P	S/P	164+2079	K091154	RBS+luxI+RBS+Mnt+TT	R/X; S/P	X/P	1137	K091155	1309

Parts

BBa_	Description	Source	location('07)	Vector	Responsible
B0015	double terminator	registry 07	Plt.1-1I;Plt.3-3O	pSB1AK3
I13025	RBS+Mnt	registry 07	Plt.1-23J	pSB1A2
K091143	plasR/cI	MW	N/A	pSB1A2
C0261	RBS+luxI	registry 07	Plt.1-24A	pSB1A2