Difference between revisions of "LasR/cI"

Latest revision as of 18:24, 22 July 2008

Goal:

pBAD+RBS+lasR+TT(James DC)

S0388?(I13453+B0034+C0079+B0015)

pLasR/cI+RBS+LuxI+RBS+Mnt+TT

K091143+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015

Ligation Plan

Parts

BBa_	Description	Source	location('07)	Vector
B0015	double terminator	registry 07	Plt.1-1I;Plt.3-3O	pSB1AK3
I13025	RBS+Mnt	registry 07	Plt.1-23J	pSB1A2
K091143	plasR/cI	MW	N/A	pSB1A2
C0261	RBS+luxI	registry 07	Plt.1-24A	pSB1A2

@@ Line 7: / Line 7: @@
 '''pLasR/cI+RBS+LuxI+RBS+Mnt+TT'''
-'''K09110?'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''
+'''K091143'''+'''C0261'''(B0034+C0061)+'''I13025'''(B0034+C0072)+'''B0015'''
 '''Ligation Plan'''
@@ Line 27: / Line 27: @@
 |'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size'''
 |-
-| 1|| K091143 ||plasR/cI ||R/X; S/P||S/P || 164+2079||C0261||RBS+luxI  ||R/X;S/P  ||X/P  ||661 ||TBD ||
+| 1|| K091141||RBS+Mnt+TT  ||R/X;S/P  ||R/X ||468+2079 ||C0261||RBS+luxI  ||R/X;S/P  ||R/X  ||661 ||K091154 ||1137
 |-
-| 2|| B0015 ||TT ||R/X; S/P||R/X || 129+2079||I13025||RBS+Mnt  ||R/X;S/P  ||R/S  ||331 ||TBD ||
+| 2||K091143||plasR/cI ||R/X;S/P ||S/P ||164+2079 ||K091154||RBS+luxI+RBS+Mnt+TT ||R/X; S/P  ||X/P  ||1137 ||K091155 ||1309
-|-
-| 3||K091141||RBS+Mnt+TT ||R/X;S/P ||R/X ||468+2079 ||TBD||plasR/cI+RBS+luxI ||R/X; S/P  ||R/S  || ||TBD ||
 |-
 |}
@@ Line 48: / Line 46: @@
 |[http://partsregistry.org/Part:BBa_I13025 I13025] ||RBS+Mnt ||registry 07  ||Plt.1-23J ||pSB1A2 ||
 |-
-|[http://partsregistry.org/Part:BBa_K09110? K09110?] ||plasR/cI ||MW ||N/A ||pSB1A2 ||
+|[http://partsregistry.org/Part:BBa_K091143 K091143] ||plasR/cI ||MW ||N/A ||pSB1A2 ||
 |-
 |[http://partsregistry.org/Part:BBa_C0261 C0261] ||RBS+luxI ||registry 07  ||Plt.1-24A ||pSB1A2 ||
 |-
 |}