Difference between revisions of "Mnt/Tet"

Revision as of 19:29, 2 July 2008

Parts Relevant to XOR Gate:

part no.	description	source	responsible
K091105	pMnt/Tet	MW '08	RC
B0034	RBS	registry	TBD
C0061	Lux gene(+LVA)	registry	TBD
C0062	Lux gene(-LVA)	registry	TBD
C0051	cI gene	registry	TBD
B0015	TT	registry	TBD
S0101	RBS+TetR	MW '08	TBD
I13453	pBAD	registry	TBD
pSB2K3	pSB2K3	registry	TBD

Steps Needed for building XOR Gate: pSB1A3

step no.	vector BBa	vector description	vector suffix prefix	vector enzymes	vector size (bp)	insert BBa	insert description	insert suffix prefix	insert enzymes	insert size (bp)	product BBa	product size
1	B0034	RBS	suffix	SP	2169	C0051	cI	suffix, prefix	XP	750	TBD	3005
2	C0261	RBS+ Lux	suffix	SP	3011	product from step 2	RBS+ cI	suffix, prefix	XP	762	TBD	3773
3	B0015	TT	prefix	RX	2278	product from step 3	RBS+Lux+ RBS+cI	suffix, prefix	RS	3612	TBD	3894
4	K091105	pMnt/Tet	suffix	SP	2255	product from step 4	RBS+Lux+RBS+cI+TT	suffix prefix	XP	3751	TBD	3992

TetR protein needed for XOR Gate: pSB2K3

step no.	vector BBa	vector description	vector suffix prefix	vector enzymes	vector size (bp)	insert BBa	insert description	insert suffix prefix	insert enzymes	insert size (bp)	product BBa	product size
1	I13453	pBAD	suffix	SP	2287	S0101	RBS+TetR	suffix, prefix	XP	703	TBD	2990
2	B0015	TT	prefix	RX	2278	product from step 1	pBAD+RBS+TetR	suffix, prefix	RS	833	TBD	3111
3	pSB2K3	kan resistance	prefix, suffix	RP	4425	product from step 2	pBAD+RBS+TetR+TT	suffix, prefix	RP	954	TBD	5379

@@ Line 47: / Line 47: @@
 !width="30"| product size
 |-
-| 1 || B0034 || RBS || suffix || SP || 2169 || C0061 || Lux (+LVA) || suffix, prefix || XP || 756 || C0261 || 3011
+| 1 || B0034 || RBS || suffix || SP || 2169 || C0051 || cI || suffix, prefix || XP || 750 || TBD || 3005
 |-
-| 2 || B0034 || RBS || suffix || SP || 2169 || C0051 || cI || suffix, prefix || XP || 750 || TBD || 3005
+| 2 || C0261 || RBS+ Lux || suffix || SP || 3011 || product from step 2 || RBS+ cI || suffix, prefix || XP || 762 || TBD || 3773
 |-
-| 3 || C0261 || RBS+ Lux || suffix || SP || 3011 || product from step 2 || RBS+ cI || suffix, prefix || XP || 762 || TBD || 3773
+| 3 || B0015 || TT || prefix || RX || 2278 || product from step 3 || RBS+Lux+ RBS+cI || suffix, prefix || RS || 3612 || TBD || 3894
 |-
-| 4 || B0015 || TT || prefix || RX || 2278 || product from step 3 || RBS+Lux+ RBS+cI || suffix, prefix || RS || 3612 || TBD || 3894
+| 4 || K091105 || pMnt/Tet || suffix || SP || 2255 || product from step 4 || RBS+Lux+RBS+cI+TT || suffix prefix || XP || 3751 ||   TBD || 3992
-|-
-| 5 || K091105 || pMnt/Tet || suffix || SP || 2255 || product from step 4 || RBS+Lux+RBS+cI+TT || suffix prefix || XP || 3751 ||   TBD || 3992
 |}