Mnt/Tet

Robert Cool

Parts Relevant to XOR Gate:

part no.	description	source	responsible
K091105	pMnt/Tet	MW '08	RC
B0034	RBS(34)	registry	RC
C0061	Lux gene(+LVA)	registry	TBD
C0062	Lux gene(-LVA)	registry	TBD
C0051	cI gene	registry	TBD
B0015	TT	registry	RC
S0101	RBS(34)+TetR	MW '08	RC
I13453	pBAD	registry	RC
pSB2K3	kan resistance	registry	TBD
S0103	RBS(34)+cI	registry	RC
C0261	RBS(34)+LuxI	registry	RC
P0455	RBS+cI+TT	registry	RC

Parts Needed for XOR Gate

part no.	description	source	responsible
K091105	pMnt/Tet	MW '08	RC
B0034	RBS(34)	registry	TBD
B0015	TT	registry	RC
S0101	RBS(34)+TetR	MW '08	RC
I13453	pBAD	registry	RC
S0103	RBS(34)+cI	registry	RC
C0261	RBS(34)+LuxI	registry	RC
P0455	RBS+cI+TT	registry	RC
pSB4A3	low copy Amp plasmid	registry	RC

Steps Needed for building XOR Gate: pSB1A3

step no.	vector BBa	vector description	vector suffix prefix	vector enzymes	vector size (bp)	insert BBa	insert description	insert suffix prefix	insert enzymes	insert size (bp)	product BBa	product size	status
1	K091105	pMnt/Tet	suffix	SP	2200	C0261	RBS+LuxI	suffix, prefix	XP	661	TBD	2861	miniprep verified (K091132)
2	B0015	TT	prefix	RX	2278	S0101	RBS+cI	suffix, prefix	RS	793	TBD	3081	same as P0455
3	product from step 1	pMnt/Tet+RBS+LuxI	suffix	SP		product from step 2	RBS+cI+TT	suffix prefix	XP		TBD		miniprep verified (K091144)

TetR protein needed for XOR Gate: pSB2K3

step no.	vector BBa	vector description	vector suffix prefix	vector enzymes	vector size (bp)	insert BBa	insert description	insert suffix prefix	insert enzymes	insert size (bp)	product BBa	product size	status
1	I13453	pBAD	suffix	SP	2287	S0101	RBS+TetR	suffix, prefix	XP	703	TBD	2990	miniprep verified (K091133)
2	B0015	TT	prefix	RX	2278	product from step 1	pBAD+RBS+TetR	suffix, prefix	RS	833	TBD	3111	miniprep verified (K091145)
3	pSB4A3	low copy amp resistance	prefix, suffix	RP	4425	product from step 2	pBAD+RBS+TetR+TT	suffix, prefix	RP	954	TBD	5379