Difference between revisions of "Mnt/Tet"

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!width="30"| status
 
!width="30"| status
 
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| 1 || K091105 || pMnt/Tet || suffix || SP || 2200 || C0261 || RBS+LuxI || suffix, prefix || XP || 661 || TBD || 2861 || miniprep not verified yet
+
| 1 || K091105 || pMnt/Tet || suffix || SP || 2200 || C0261 || RBS+LuxI || suffix, prefix || XP || 661 || TBD || 2861 || miniprep verified (K091132)
 
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|-
 
| 2 || B0015 || TT || prefix || RX || 2278 || S0101 || RBS+cI || suffix, prefix || RS || 793 || TBD || 3081 || same as P0455
 
| 2 || B0015 || TT || prefix || RX || 2278 || S0101 || RBS+cI || suffix, prefix || RS || 793 || TBD || 3081 || same as P0455
 
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|-
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| 3 || product from step 1 || pMnt/Tet+RBS+LuxI || suffix || SP ||  || product from step 2 || RBS+cI+TT || suffix prefix || XP ||  ||  TBD || ||  
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| 3 || product from step 1 || pMnt/Tet+RBS+LuxI || suffix || SP ||  || product from step 2 || RBS+cI+TT || suffix prefix || XP ||  ||  TBD || || cells growing 
 
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!width="30"|status
 
!width="30"|status
 
|-
 
|-
−
| 1 || I13453 || pBAD || suffix || SP || 2287 || S0101 || RBS+TetR || suffix, prefix || XP || 703 || TBD || 2990 || miniprep not verified yet
+
| 1 || I13453 || pBAD || suffix || SP || 2287 || S0101 || RBS+TetR || suffix, prefix || XP || 703 || TBD || 2990 || miniprep verified (K091133)
 
|-
 
|-
−
| 2 || B0015 || TT || prefix || RX || 2278 || product from step 1 || pBAD+RBS+TetR || suffix, prefix || RS || 833 || TBD || 3111 ||
+
| 2 || B0015 || TT || prefix || RX || 2278 || product from step 1 || pBAD+RBS+TetR || suffix, prefix || RS || 833 || TBD || 3111 || cells growing
 
|-
 
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| 3 || pSB2K3 || kan resistance || prefix, suffix || RP || 4425 || product from step 2 || pBAD+RBS+TetR+TT || suffix, prefix || RP || 954 || TBD || 5379||
 
| 3 || pSB2K3 || kan resistance || prefix, suffix || RP || 4425 || product from step 2 || pBAD+RBS+TetR+TT || suffix, prefix || RP || 954 || TBD || 5379||
 
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Revision as of 16:19, 15 July 2008

Robert Cool

Parts Relevant to XOR Gate:

part no. description source responsible
K091105 pMnt/Tet MW '08 RC
B0034 RBS(34) registry TBD
C0061 Lux gene(+LVA) registry TBD
C0062 Lux gene(-LVA) registry TBD
C0051 cI gene registry TBD
B0015 TT registry TBD
S0101 RBS(34)+TetR MW '08 TBD
I13453 pBAD registry TBD
pSB2K3 kan resistance registry TBD
S0103 RBS(34)+cI registry TBD
C0261 RBS(34)+LuxI registry TBD

Parts Needed for XOR Gate

part no. description source responsible
K091105 pMnt/Tet MW '08 RC
B0034 RBS(34) registry TBD
B0015 TT registry TBD
S0101 RBS(34)+TetR MW '08 TBD
I13453 pBAD registry TBD
S0103 RBS(34)+cI registry TBD
C0261 RBS(34)+LuxI registry TBD
low or mid copy Amp plasmid
low or mid copy Kan plasmid

Steps Needed for building XOR Gate: pSB1A3

step no. vector BBa vector description vector suffix prefix vector enzymes vector size (bp) insert BBa insert description insert suffix prefix insert enzymes insert size (bp) product BBa product size status
1 K091105 pMnt/Tet suffix SP 2200 C0261 RBS+LuxI suffix, prefix XP 661 TBD 2861 miniprep verified (K091132)
2 B0015 TT prefix RX 2278 S0101 RBS+cI suffix, prefix RS 793 TBD 3081 same as P0455
3 product from step 1 pMnt/Tet+RBS+LuxI suffix SP product from step 2 RBS+cI+TT suffix prefix XP TBD cells growing


TetR protein needed for XOR Gate: pSB2K3

step no. vector BBa vector description vector suffix prefix vector enzymes vector size (bp) insert BBa insert description insert suffix prefix insert enzymes insert size (bp) product BBa product size status
1 I13453 pBAD suffix SP 2287 S0101 RBS+TetR suffix, prefix XP 703 TBD 2990 miniprep verified (K091133)
2 B0015 TT prefix RX 2278 product from step 1 pBAD+RBS+TetR suffix, prefix RS 833 TBD 3111 cells growing
3 pSB2K3 kan resistance prefix, suffix RP 4425 product from step 2 pBAD+RBS+TetR+TT suffix, prefix RP 954 TBD 5379
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