J-GGA Scaffold 2.0 Sequence

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Scaffold 2.0 Top

(5’to 3’)

AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGGTCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA

Scaffold 2.0 Bottom

(5’to 3’)

GCGGCCGCTACTAGTAGCTGTCTGTTCGTTAGGCCTAATCGCACAGTCATTAGCACTCCGCTCCTGGTTCAAGACCCAACGTGGTCCTCTGTGTATACGGCTCTAGAAGCGGCCGCG


Annealed Scaffold Top and Bottom Oligos 

  Prefix          Junction A               Junction B               Junction C              Junction D         suffix



AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGGTCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA

    GCGCCGGCGAAGATCTCGGCATATGTGTCTCCTGGTGCAACCCAGAACTTGGTCCTCGCCTCACGATTACTGACACGCTAATCCGGATTGCTTGTCTGTCGATGATCATCGCCGGCG


Scaffold 2.0 Ordering

Scaffold2.0_Top1; AATTCGCGGCCGCTTCTAGAGCCGTATACACAGAGGACCACGTTGGG

Scaffold2.0_Top2; TCTTGAACCAGGAGCGGAGTGCTAATGACTGTGCGATTAGGCCTAACGAACAGACAGCTACTAGTAGCGGCCGCTGCA

Scaffold2.0_Bottom1; GCGGCCGCTACTAGTAGCTGTCTGTTCGTTAGGCCTAATCGCAC

Scaffold2.0_Bottom2; AGTCATTAGCACTCCGCTCCTGGTTCAAGACCCAACGTGGTCCTCTGTGTATACGGCTCTAGAAGCGGCCGCG

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