Primer Design for the RFP

From GcatWiki
Revision as of 17:11, 30 July 2013 by Spolpitiyaarachchige (talk | contribs) (Created page with "RFP for scaffold 2.0 Forward primer will bind to 5’ end of RFP JGGA2.0_RFP_for; GCATGGTCTCTGCGAGCTTCCTCCGAAGACGTTATC Reverse primer will to 3’ end of RFP JGGA2.0_RFP_r...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

RFP for scaffold 2.0

Forward primer will bind to 5’ end of RFP

JGGA2.0_RFP_for; GCATGGTCTCTGCGAGCTTCCTCCGAAGACGTTATC

Reverse primer will to 3’ end of RFP

JGGA2.0_RFP_rev; GCATGGTCTCAAGGCTTATTAAGCACCGGTGGAG

Primers for RFP Insertion between Junctions C and D

Oligos to be Ordered

JGGA2.0_RFP_for; GCATGGTCTCTGCGAGCTTCCTCCGAAGACGTTATC

JGGA2.0_RFP_rev; GCATGGTCTCAAGGCTTATTAAGCACCGGTGGAG


>BBa_E1010 Part-only sequence (706 bp)

Atg gct tcc tcc gaa gac gtt atc aaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtg

aaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagta

cggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaa

gacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtc

cggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaact

gaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggac

atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca

ctccaccggtgcttaataa

Retrieved from "http://gcat.davidson.edu/GcatWiki/index.php?title=Primer_Design_for_the_RFP&oldid=16592"