Mnt/Tet

Parts Relevant to XOR Gate:

part no.	description	source	responsible
K091105	pMnt/Tet	MW '08	RC
B0034	RBS	registry	?
C0061	Lux gene(+LVA)	registry	?
C0062	Lux gene(-LVA)	registry	?
C0051	cI gene	registry	?
B0015	TT	registry	?

Steps Needed for building XOR Gate: pSB1A3

step no.	vector BBa	vector description	vector suffix prefix	vector enzymes	vector size (bp)	insert BBa	insert description	insert suffix prefix	insert enzymes	insert size (bp)	product BBa	product size
1	B0034	RBS	suffix	SP	2169	C0061	Lux (+LVA)	suffix, prefix	XP	756	?	3011
2	B0034	RBS	suffix	SP	2169	C0051	cI	suffix, prefix	XP	750	?	3005
3	product from step 1	RBS+ Lux	suffix	SP	3011	product from step 2	RBS+ cI	suffix, prefix	XP	762	?	3773
4	B0015	TT	prefix	RX	2278	product from step 3	RBS+Lux+ RBS+cI	suffix, prefix	RS	3612	?	3894
5	K091105	pMnt/Tet	suffix	SP	2255	product from step 4	RBS+Lux+RBS+cI+TT	suffix prefix	XP	3751	?	3992

TetR protein needed for XOR Gate: pSB2K3

step no.	vector BBa	vector description	vector suffix prefix	vector enzymes	vector size (bp)	insert BBa	insert description	insert suffix prefix	insert enzymes	insert size (bp)	product BBa	product size
1	I13453	pBAD	suffix	SP	2287	S0101	RBS+TetR	suffix, prefix	XP	703	?	2990
2	B0015	TT	prefix	RX	2278	product from step 1	pBAD+RBS+TetR	suffix, prefix	RS	833	?	3111
3	pSB2K3	kan resistance	prefix, suffix	RP	4425	product from step 2	pBAD+RBS+TetR+TT	suffix, prefix	RP	954	?	5379