Mnt/Lac

Parts Relevant to XOR Gate:

part no.	description	source	responsible
K091104	pMnt/Lac	MW '08	AA
B0034	RBS	registry	"
P0455	RBS+cI+TT	registry	"
K091109	LuxS +AI-2	MW '08	"
C0012	LacI (+LVA)	DC '08	"
B0015	TT	registry	"
I13453	pBAD	registry	"

Goal:

pBAD+RBS+LacI+TT+pMnt/Lac+RBS+LuxS+RBS+cI+TT

I13453 + B0034+ C0012 + B0015 + K091104 + B0034 + K091109 + P0455 (B0034+C0051+B0015)

Ligation Plan

	Vector					Insert					Product
Step	BBa_	Description	Pre-/suffix	Enzymes	Size bp	BBa_	Description	Pre-/suffix	Enzymes	Size bp	BBa_	Size
1	B0034	RBS	R/X; S/P	S/P	12	I13453	pBAD	R/X;S/P	X/P	120	TBD
2	TBD	pBAD+RBS	R/X;S/P	R/X		C0012	LacI	R/X; S/P	R/S	1128	TBD
3	TBD	pBAD+RBS+LacI	R/X; S/P	S/P	1128	B0015	TT	R/X; S/ P	X/P	129	TBD
4	TBD	pBAD+RBS+LacI+TT	R/X; S/P	R/X	TBD	K091104	pMnt/Lac	R/X; S/P	R/S	87	TBD
5	TBD	pBAD+RBS+LacI+TT+pMnt/Lac	R/X; S/P	S/P	TBD	B0034	RBS	R/X;S/P	R/S	12	TBD
6	TBD	pBAD+RBS+LacI+TT+pMnt/Lac+RBS	R/X; S/P	S/P	TBD	K091109	LuxS	R/X; S/P	X/P	516	TBD
7	TBD	pBAD+RBS+LacI+TT+pMnt/Lac+RBS+LuxS	R/X; S/P	S/P	TBD	P0455	RBS+cI+TT	R/X; S/P	X/P	896	TBD