Mnt/Lac
From GcatWiki
Parts Relevant to XOR Gate:
part no. | description | source | responsible |
---|---|---|---|
K091104 | pMnt/Lac | MW '08 | AA |
B0034 | RBS | registry | " |
P0455 | RBS+cI+TT | registry | " |
K091109 | LuxS +AI-2 | MW '08 | " |
C0012 | LacI (+LVA) | DC '08 | " |
B0015 | TT | registry | " |
I13453 | pBAD | registry | " |
Goal:
pBAD+RBS+LacI+TT+pMnt/Lac+RBS+LuxS+RBS+cI+TT
I13453 + B0034+ C0012 + B0015 + K091104 + B0034 + K091109 + P0455 (B0034+C0051+B0015)
Ligation Plan
Vector | Insert | Product | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Step | BBa_ | Description | Pre-/suffix | Enzymes | Size bp | BBa_ | Description | Pre-/suffix | Enzymes | Size bp | BBa_ | Size |
1 | B0034 | RBS | R/X; S/P | S/P | 12 | I13453 | pBAD | R/X;S/P | X/P | 120 | TBD | |
2 | TBD | pBAD+RBS | R/X;S/P | R/X | C0012 | LacI | R/X; S/P | R/S | 1128 | TBD | ||
3 | TBD | pBAD+RBS+LacI | R/X; S/P | S/P | 1128 | B0015 | TT | R/X; S/ P | X/P | 129 | TBD | |
4 | TBD | pBAD+RBS+LacI+TT | R/X; S/P | R/X | TBD | K091104 | pMnt/Lac | R/X; S/P | R/S | 87 | TBD | |
5 | TBD | pBAD+RBS+LacI+TT+pMnt/Lac | R/X; S/P | S/P | TBD | B0034 | RBS | R/X;S/P | R/S | 12 | TBD | |
6 | TBD | pBAD+RBS+LacI+TT+pMnt/Lac+RBS | R/X; S/P | S/P | TBD | K091109 | LuxS | R/X; S/P | X/P | 516 | TBD | |
7 | TBD | pBAD+RBS+LacI+TT+pMnt/Lac+RBS+LuxS | R/X; S/P | S/P | TBD | P0455 | RBS+cI+TT | R/X; S/P | X/P | 896 | TBD |