2/8/18
Clicking on i button lets us see what parameters we entered when running test
P-values: -adjuststed p-value wont work because our samples are so small -we will use regular p-value (cutoff <0.05)
Test:
-DEseq
---All C201R vs all wildtype (both male and female)
link:
[https://docs.google.com/spreadsheets/d/1aqRSoBKsdD7N9NNgYIWdH-uLPmGy_y3Wm94tVvXbLso/edit?ts=5a7c661a#gid=0
]
Today- -sort by p value FNTM-mouse specific Gene cards Gene Weaver Go rilla FNTM String
minimum fold change: ~2 needed
How our data was collected: whole spinal cord
reasons why we might get genes that dont have anything to do with CMT -non-nueronal tissue -genes
impression: -protein is secreted and blocking signaling -surface protein,
reason why we don't have tighter clustering: -receptors not completely BLOCKED, and thats part of the disease progression -baseline genetic differences related NOT related to CMT