Archaeal BJ1 Virus Hit from Spacer 2 in Crispr One: Blast Alignments to Other Halophiles

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What does this virus primarily attack? (Blasted the virus against nr/nt database)

Avbj1.jpg

Infects other halophiles, other bacteria and even other viruses


Blastn Alignment: Query= viral (Accession Number: AM419438.1) and Subject=species of choice


OUR SPECIES: Accession Number: NC_013202

Below are the hits which insert with spacers in our species genome:

Only see one hit with the virus in one of the spacers in our species. . . aka this virus to our knowledge only inserts into one spacer. . . Note that it only inserts in the spacer region and not the DR region and that the spacer it inserts in corresponds with the blasted spacer that gave this virus as a hit

OurspeciesOne.jpg



CONCLUSION: THESE VIRAL INSERTIONS ARE NOT INTO SPACERS IN THIS SPECIES: aligned spacer number with the significant hits below


Halorhabdus utahensis: Accession number determined from NCBI: NC_013158.1

Lots of hits, the ones shown below have e-values less than 0.005, the next e-value not included is 0.018

HU1.jpg

HU2.jpg

HU3.jpg

HU4.jpg

Check to see if any of these hits occur in the spacers of this species by using crispr finder and the nucleotide base numbers


Halobacterium salinarium R1: Accession number determined from NCBI: NC_010364

Lots of hits, the ones shown have e-values less than 0.003, next e-value hit not shown is 0.011

HS1.jpg

HS2.jpg

HS3.jpg

HS4.jpg

Check to see if any of these hits occur in the spacers of this species by using crispr finder and the nucleotide base numbers


Haloarcula vallismortis Blast Results

didnt accept anything above 0.009

blast recall number: G5P5M4WY111



Haloarcula sinaiiensis: Blast Results

CONCLUSION: found viral insertions of this virus in some of the spacers

didnt accept anything above 0.009

blast recall number: G5RXZPH1113