Citrate Synthase
I did a blastp search using our species' citrate synthase protein sequence.
The following halophillic Archaea were the top hits and are accompanied by their basltp results:
Haloarcula marismortui citrate synthase protein sequence:
1 msddlkkgle gvivaesels vidgdagklv yrgytiedla kgasyeevly llwhghlpnr
61 delsefkqam vdargvdddv istvrqlaea denpmaalrt avsmlsafdp apedaeptde
121 mvnletgrri takiptiiaa ftrirdgkep vephddldha anflymlnge epddvladvf
181 dqalvlhadh glnastfsai ttastlsdvh savtsaigtl kgplhgganq dvmemlkevd
241 daesdpldwv knaldegrrv sgfghrvynv kdprakilge rskelgeaag tlkwyemstt
301 iedylmeekg lapnvdfysa styyqmgipi diytpifams rvggwvahvf eyiednrlir
361 praryvgknt detefvplde r
Natronomonas pharaonis - halophillic archaean - got rid of because is not just a salt-loving organism but can also survive high salinity citrate synthase protein sequence:
1 msdelkkgle gvlvaessls yidgdegklv yrgytiddla rnasfeevvy llwhgelpta
61 delgsfkssm aeerhlddgv qqlvrelaea deepmaalrs ivsslsaydp dadaeptdea
121 anlrkgrrit akiptalaaf sriregddpv epredldhaa nflymlngee pdevladtfd
181 malvlhadhg lnastfsamv tsstladlha aitsavgals gslhgganqd vmeallelde
241 sgkepvewve drldagervp gfghrvynvk dprakilsee sealgeaagt pqwhnystei
301 eeylteekgl apnvdfysas tyyqmgipvd iytpifamsr vggwiahvle qyddnrlirp
361 rgryvgpddr tvtpider
Halogeometricum borinquense citrate synthase protein sequence
1 msddlkrgle gvlvaesels yidgdagqlv yrghtiedla rnasyeevvy llwhgelptr
61 eqldefstam aeerelddav ldeiralaaa devpmaalrt ivsslsaydd dadhedvtdl
121 ktnikkgcri takiptalaa fnrlrngedv vhprtdlsha anflymlnge epddvlaetf
181 dmalvlhadh glnastfsam vtastlsdlh savtsaigtl agslhggana nvmrmlkeid
241 esdkdptqwv edalesgrrv pgfghrvynv kdprakilge ksealgeaag dmkwydysva
301 iedyigeekg lapnvdfysa styyqmgipv diytpifams rvggwiahvi eqfednrlir
361 praryvgred qtftpider
Haloferax volcanii citrate synthase protein sequence
1 msgelkrgle gvlvaeskls fidgdagqlv ycgydiedla rdasyeevly llwhgalptg
61 eeldafsdel aahrdlddgv ldvarelaeq despmaalrt lvsamsayde sadfedvtdr
121 evnlekakri takmpsvlaa yarfrrgddy vepdeslnha anflymlnge epnevlaetf
181 dmalvlhadh glnastfsam vtsstlsdly savtsaigtl sgslhggana nvmrmlkdvd
241 dsdmdptewv kdaldrgerv agfvhrvynv kdprakilga ksealgeaag dmkwyemsva
301 ieeyigeekg lapnvdfysa styyqmgipi dlytpifavs raggwiahvl eqyednrlir
361 prarytgekd ldftpvder
Haloquadratum walsbyi citrate synthase protein sequence
1 msdevkrgle dvlvaesels cidgdagelv yrgysiedla qkasyeevvy llwhgrlptg
61 delqsftdam ieertintgv yeqierlaaa despmaalrt ivstlsaydp dadaevidhe
121 anarkarria aklptalaal qrcregndpv tprsdldhaa nflymlngse pdpvlaetfd
181 malvlhadhg lnastfsakv tastlsdlhc aittavgtls gslhgganan vmrmlkevha
241 sdadavtwie dalesgrrvp gfghrvynvk dprarilger skelaeaage mcwynysiti
301 eeyiddhkgl apnvdfysas tyyqmgipid iytpifamar tagwiahtfe qydenrlirp
361 raryvgneda tfksvekr
Halobacterium salinarum citrate synthase protein sequence:
1 msdelkkgle dvlvaesdls fidgdagklv ycgyaiedla rtasyeevly llwhgdlptr
61 seldafradm ashreidddv lalvenlaaa dadpmaalrt aasalsahdd taggsdpdda
121 resgkritak lptivaaftr lrngddvvap rddlghaanf lymlngeapd dvladvfdqa
181 lvlhadhgln astfsgmvta stladvhaav tsaigtlsgs lhggananvm rmlkevddsd
241 kdplswvqda langervmgf ghrvynvkdp rahilserse elgaaagdtk wyemsvaied
301 ymadekglap nvdfysasty yqmgipvdiy tpifamsrvg gwvghvleqy ddnrlirpra
361 ryvgaedrds tplder
Halorhabdus utahensis
1 mpdelrkgle gvlvaessls didgeegrlv yrgyaiedla rntsfeevly llweghlpte
61 selepfeeqm aeeravreev lstvrelaaa eenpmaalrt avsmlsaydp ddstgeagdg
121 eiaqrkgrri takmptivaa ykrlrdgeep vaprtdlsha enflymlnge epaqtladvf
181 dmalvvhadh ginastfaam vtastlsdlh ssvtsaigal kgglhgganq nvmqmlleld
241 esdltavewa reavesgdri pgfghrvydv kdprarilsa kskalgkaad elkwysysra
301 ieefmaketg iapnvdfysa smyyemgipi dlytpifams rvggwvahvl eyqeenrlir
361 praryvgped refvrieqr
Halorubrum lacusprofundi
>Halorubrum lacusprofundi ATCC 49239 MSDELKRGLEGVLVAESDLSYVDGEVGKLVYRGYDIEDLARGASYEEVLYLLWRGSLPTREELDAFTADL AAERAVDDDALDAVRTLADAGERPMAALRTAVSMLSAYEPESDADPEDLDATLRQGRRITAKIPTLLAAF ERARQGEDPVAPDPDLSHAANFLYMLTGTEPDDVSAETFDMALTLHADHGLNASTFTAIVIGSTMADVYS GVTGGIGALSGPLHGGANQDVMEVLQEVDASDKDPVQWVKDAREEGRRIPGFGHRVYKVKDPRAKILEEK LRDLSESSGDTKWLDYTTAIEEYLTEQGLLDKGIAPNVDFYSGSVYDSLGIPVDMYTPIFAMSRAGGWIA HMVEYQEDNRLIRPRARYTGPKASEFVPVDER
The following are salt-loving bacertia with top hits
Salinibacter ruber- Extremely halophilic, strictly aerobic, red-pigmented bacterium citrate synthase protein sequence
1 mllcsektpa wagprgllqk rsradslnag lsqrpaclrp cpfgrdghav siplrlspht
61 ffdmadqesv telttdkapi karglegvia leselssidg dkgqliyrgy dihdladhas
121 feevvyllrh gelptqseld dfsshlrser alpapilnll retpadahpm avlrtavsal
181 alydeeadtm eeaanhrkad rilaqiptii aafdrlrkdk epvapldhgs mahnflymln
241 gekpgaaaed tfdvclvlha dhglnastft srvigstlsd mysavsgsvg alkgrlhgga
301 nqevmrmlld idekgadpts yirdrldade rvmgfghrvy ntmdpraail rtmvkelsee
361 agdmkwykyt tkihetmtee tgidpnvdff sgptyyqlgl dpdlfttifa lsrtagwtah
421 lleqwadnrl irpraqyvga rdrtvapiae r
Bacillus selenitireducens- haloalkaliphilic bacerium- throwing this out because i want to focus in on salt-loving specifically and do not want to have other extreme natured organisms in the salt-loving category citrate protein sequence
1 msttkglegv vattssvssi iddvltyrgy niddladhas feevvyllwn eklptkqeld
61 tfkkelaevs avpdevinqm rtypidqvhp maalrtavsn lalfdeeads hdedanrrka
121 lklqaqlpti vaafarvrqg kdpvapkqel syaanflyml ngeepdetsv kaidkalvlh
181 adhelnastf tsrvcvatls dmysgitaai galkgplhgg anervmamls eietvenaep
241 yikealankv kimgfghrvy kngdprakhl kemsrqmtei tgeskwydms vkvdeivtge
301 kgllpnvdfy sasvyhslgi dhdlftpifa vsrvsgwiah ileqysnnrl irpraeyvgp
361 dkqswipiek r
ClustalW Alignment of Salt-Loving Organisms:
The following is a salt-tolerant bacteria:
Oceanobacillus iheyensis citrate synthase protein sequence:
1 mtastkgleg ivatqssiss iiddqlsyvg yriddlaens sfeeiiyllw nqklpnksel
61 delksqlaen mdipdaiinh lrsydlntvh pmaalrtais llglydnead vmdeqankrk
121 avriqaqiat ivttfsrirk gqepvkpkkd lsyaenfiym lngkepedie veainkalvl
181 hadhelnast ftarvcvatl sdiysgitaa igalkgplhg ganenvmkml teigeeenai
241 pyikeklank dkimgmghrv yrtgdprakf lremskeltk ltgqekwynm svkiedyike
301 nkglpanvdf ysasvyhslg ldhdiftplf avsrtsgwia hileqyennr lirpraeytg
361 pelrdyvsie qrg
The following non-salt loving prokaryotes were the top hits among their domain
Alicyclobacillus acidocaldarius- thermoacidiphillic bacterium citrate synthase protein sequence:
1 maaettfkag ledvvsntse icfldgkqgr llyygydihd lvdggasfee vvyllwhgel
61 pnqselkaft eelaserala epvldllkrl pkdanamavl rtavsflgly dpddgdesle
121 anyrkaarlv akiptivtsy erirqgldpv qpdpslsaaa nffyllrgtk psefeekafn
181 talilhadhe lnastfsarv tagtlsdmys aitsaigtlk gplhgganeq vmrmlleigd
241 pskaitwide alaqkkkimg fghrvyrted prathlrels kqagelkget kwfemsqaie
301 khmlevkglh anvdfysasl yyslgipthl ytpifacsri sgwtahvleq yknnrlirpr
361 aeyvgptdrk fvplser
Acidobacterium capsulatum- aerobic, mesophilic, chemo-organotroph bacterium citrate synthase protein sequence
1 mstavatkgl egivaansgi cwidgdagvl ayrgidihel athsnfeett yllwhgklpt
61 qaeladfskk laaarridpk iydllrslpk tgtpmealrt avsalsffdp neravdhdsn
121 vrkafdltaq iamivaafdr irkgkaivep dpslshagnf lymlngekps etatkafdia
181 lilhadheln astfaarvia atlsdihsai tgaigalkgp lhgganeavm rmlfeiekag
241 adpvehvkkm laekkkvsgf ghrvyhtedp rathlrrmse dlgrsannak wfemsrkiel
301 yinqekklna nvdfysasty ttlgididlf tpifaisria gwsahvieql ddnrlirpra
361 eyigpeypsk yipvaer
Bacillus cereus-endemic, soil-dwelling citrate synthase protein sequence
1 mgefsgkgen vmtvirgleg vvattssvss iiddtltyvg yniddlaena tfeevvyllw
61 hrklpnekel aefkdtvsey ykvpgeilty lkqvdlkiah pmsvlrtais mlslydesae
121 imdgksnylk avklqaqvgt liaayarirk gldvveprkd lslaanflym lndrepneve
181 ieafdkalvl hadhelnast ftarvcvatl sdvysgitaa igalkgplhg ganenvmkml
241 teigeeenve syihnalqnk vkimgfghrv yehgdprakh lremskrlcv llgedkwynm
301 sikiedivtk ekglppnvdf ysasvyhclg idhdlftpif aisrmsgwla hileqyennr
361 lirpradyng pthqvyvpia qr
Bacillus thuringiensis- soil-dwelling bacterium, commonly used as a pesticide citrate synthase protein sequence
1 mgefsgkgen vmtvirgleg vvattssvss iiddtltyvg yniddlaena tfeevvyllw
61 hrklpnekel aefneivsey ykvpgeilty lkqvdlkvah pmsvlrtais mlslydesae
121 imdeksnylk avklqaqvgt lvaayarirk gldiveprkd lslaanflym lnnrepneve
181 ieafdkalvl hadhelnast ftarvcvatl sdvysgitaa igalkgplhg ganenvmkml
241 teigeeenve syihnalqnk vkimgfghrv yeqgdprakh lremskrlcv llgedkwynm
301 sikiedivtk ekglppnvdf ysasvyhclg idhdlftpif aisrmsgwla hileqyennr
361 lirpradyng pthqvyvpia qr
Bacillus weihenstephanensis-non-pathogenic, Gram-positive, typical facultative psychrophile generally found in soil citrate synthase protein sequence:
1 mtvirglegv vattssvssi iddtltyvgy niddlaenat feevvyllwh rklpnekela
61 efsgtvseyy kvpgevltyl kqvdlkiahp msvlrtaism lslydesaei mdgksnylka
121 vklqaqvgtl iaayarirkg ldvveprkdl slaanflyml ndrepnevei eafdkalvlh
181 adhelnastf tarvcvatls dvysgitaai galkgplhgg anenvmkmlt eigeeenves
241 yihnalqnkv kimgfghrvy ehgdprakhl remskrlcvl lgedkwynms ikiedivtke
301 kglppnvdfy sasvyhclgi dhdlftpifa isrmsgwlah ileqyennrl irpradydgp
361 thqvyvplaq r
Bacillus mycoides- soil dwelling citrate synthase protein sequence:
1 mgefsgkgen vmtvirgleg vvattssvss iiddtltyvg yniddlaena tfeevvyllw
61 hrklpnekel aefsgtvsey ykvpgevlty lkqvdlkiah pmsvlrtais mlslydesae
121 imdgksnylk avklqaqvgt liaayarirk gldvveprkd lslaanflym lndrepneve
181 ieafdkalvl hadhelnast ftarvcvatl sdvysgitaa igalkgplhg ganenvmkml
241 teigeeenve syihnalqnk vkimgfghrv yehgdprakh lremskrlcv llgedkwynm
301 sikiedivtk ekglppnvdf ysasvyhclg idhdlftpif aisrmsgwla hileqyennr
361 lirpradydg pthqvyvpla qr
Geobacillus sp.- thermophilic bacterium citrate synthase protein sequence:
Bacillus anthracis citrate synthase protein sequence:
1 mtvirglegv vattssvssi iddtltyagy niddlaenat feevvyllwh rklpneqela
61 kfneivseyy kvpgeilayl kqvdlkiahp msvlrtaism lslydesaei mdeksnylka
121 vklqaqvgtl vaayarirkg ldvveprndl slaanflyml ndrepnevei eafdkalvlh
181 adhelnastf tarvcvatls dvysgitaai galkgplhgg anenvmkmlt eigeeenves
241 yihnalqnkv kimgfghrvy eqgdprakhl remskrlcvl lgeekwynms ikiedivtke
301 kglppnvdfy sasvyhclgi dhdlftpifa isrmsgwlah ileqyennrl irpradyngp
361 thqvyvpiaq r
Bacillus methanolicus citrate synthase protein sequence:
Bacillus subtilis citrate synthase protein sequence:
1 mtatrglegv vattssvssi iddtltyvgy diddltenas feeiiyllwh lrlpnkkele
61 elkqqlakea avpqeiiehf ksyslenvhp maalrtaisl lglldseadt mnpeanyrka
121 irlqakvpgl vaafsrirkg lepvepredy giaenflytl ngeepspiev eafnkalilh
181 adhelnastf tarvcvatls diysgitaai galkgplhgg anegvmkmlt eigevenaep
241 yiraklekke kimgfghrvy khgdprakhl kemskrltnl tgeskwyems iriedivtse
301 kklppnvdfy sasvyhslgi dhdlftpifa vsrmsgwlah ileqydnnrl irpradytgp
361 dkqkfvpiee ra
Exiguobacterium
>Exiguobacterium sp. AT1b] MSTTKGLEGIVAAASKVSSIIDGQLTYGGYTIDDLADNATFEEVVFLLWNDRLPNAEELTALSEALVKEA TLPEEVIDTLKRAPKSANAMATIRTALSQLALYDEESEDMSDEANMRKAIRLQAQVATIVTAFARLRKGE QPVAPKPGLSYAANFLYMLNGEEPSDVAEKTIDKALILHADHELNASTFTARVCVATLSDMYSGVTAAMG ALKGPLHGGANEAVMKMLHEIDSVDRVEEYVRGKFERKEKIMGFGHRVYKDGDPRAKHLKEMSRQLTAQI GEPKWYEMSEKIDHLVETEKGLKPNVDFYSASTYYALGLEEDLFTPIFAVSRMSGWIAHILEQYADNRLI RPRAEYVGETSRKYVPVNER
I next did a ClustalW Multiple Alignment with the salt-loving and non-salt loving organisms. I was looking for areas of commonality in the salt-loving alignment that had disappeared in the combined alignment. If the conserved region disappeared than it would signal that some aspect of the citrate synthase protein sequence that is unique to halophilic organisms is not seen in non-salt loving organisms. I found common sequences in all 7 strains according to the ClustalW multiple alignment of the salt-loving organisms and then went in search for the same sequences in the overall alignment. The picture below shows a highly conserved sequence among salt loving and non salt loving organisms BUT there is one amino acid difference, a change from glycine (salt loving) to glutamic acid (non salt loving). These amino acids are highly different: glutamic acid is hydrophilic and charged whereas glycine is nonpolar and hydrophobic. This change may indicate that there is a genetic and primary protein sequence reason behind why citrate synthase needs salt to work properly.
