DM Notes 3.10.16
Today: working on presentation, approach one. Convert online presentation to powerpoint, put it on a flash drive please -Dr. C
Working on approach one:
PURPOSE: to find a small group of differentially expressed genes and use their GO IDs to uncover molecular function PROCESS: From .results files, compare gene FPKM values across samples (1-3 vs 4-6) to find most differentially expressed genes Group significant genes by GO terms to gain insight into key functions Can also compare our results to DEseq generated genes
We're working on coding/excel-ing this today. In Python, we will open up three .results files at a time. For each contig in the file, we'll generate an average FPKM (normalized) score, then output the contig and score to a text file. Back to home Dylan Maghini