Difference between revisions of "Davidson Missouri W/MWSU protocols"

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[[Diagnostic RP Digestion for Checking Insert Size]]
 
[[Diagnostic RP Digestion for Checking Insert Size]]
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[[DNA Sequencing at Iowa State University]]
  
 
[[BioBrick Digestions for Fragment and Vector Preparation]]
 
[[BioBrick Digestions for Fragment and Vector Preparation]]
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 +
[[Reducing Background from Double Digested Vector]]
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 +
[[Pouring an Agarose Gel]]
  
 
[[Fragment Purification]]
 
[[Fragment Purification]]
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 +
[[Ethanol Precipitation of Vector DNA]]
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 +
[[BioBrick Ligations]]
  
 
[[Ligation and Transformation]]
 
[[Ligation and Transformation]]
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[[Standard PCR]]
 
[[Standard PCR]]
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[[Isolation of Genomic DNA from Bacteria]]
  
 
[[Resuspending Oligos]]
 
[[Resuspending Oligos]]

Latest revision as of 14:38, 8 September 2010

What to do with a new clone

Diagnostic RP Digestion for Checking Insert Size

DNA Sequencing at Iowa State University

BioBrick Digestions for Fragment and Vector Preparation

Reducing Background from Double Digested Vector

Pouring an Agarose Gel

Fragment Purification

Ethanol Precipitation of Vector DNA

BioBrick Ligations

Ligation and Transformation

Colony PCR

Standard PCR

Isolation of Genomic DNA from Bacteria

Resuspending Oligos

Direct Synthesis with Overlapping Oligos

Bacterial Media

Measuring Fluorescence in Bacteria