Resuspending Oligos

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  A. Oligos used as both templates and primers.

add pure dH2O for 10 ug/ul (e.g. 2.10mg/210ul)

Dilute 100X to 100ng/ul

use 1 ul in 20 ul PCR

  B. Oligos used as primers only

add pure dH20 for 200 pmol/ul (e.g. 19.5 nmols= 19500 pmols; 19500 pmol/ 200 pmol/ul= 97.5 ul) label as 200 pmol/ul, freeze

dilute 10X to 20 pmol/ul

use 1 ul in 20 ul PCR