Design of C dog oligos in J-GGA Scaffold for Scaffold 2.0

C dog for scaffold 2.0

BD18 (medium)

>BBa_J119024 Part-only sequence (88 bp)

gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcgacggagcgtttctaatg

Oligos to be Ordered

BD18_top; GGAGGGGCCCAAGTTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCATGCGACGGAGCG

BD18_bot; GCACCGCTCCGTCGCATGATTAAGATGTTTCAGTACGAAAATTGCTTTCATTGTTGATCTCCTTTTTAAGTGAACTTGGGCCC

Annealed

BD18_ GGAGGGGCCCAAGTTCACTTAAAAAGGAGATCAACAATGAAAGCAATTTTCGTACTGAAACATCTTAATCATGCGACGGAGCG

        CCCGGGTTCAAGTGAATTTTTCCTCTAGTTGTTACTTTCGTTAAAAGCATGACTTTGTAGAATTAGTACGCTGCCTCGCCACG