Figure 1. Schematic representation of AND gate.

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Figure 1: Schematic representation of AND gate.

Input 1: P(sal) controls the expression supD gene, and is activated by the addition of salicylate.

Input 2: P(BAD) controls T7 RNA polymerase gene, and is activated by the addition of arabinose. Because a strong RBS for T7ptag resulted in high levels of basal expression, the RBS was mutagenized and tuned by screening for the presence of output only when both inducers were present.

Output: GFPmut3_LAA (fast-folding GFP with a degradation tag)

AND gate fig 1.jpg

(Image from Figure 1 of Anderson et al., 2007. Permission pending.)