Future Design/ 8+ HPP

Idea:

We were thinking that if we could get one strain of E.coli that is auxotrophic to all 20 amino acids we could split the amino acids and then follow our designed protocol and solve a 20 HPP at one time.

However, we could not find any strains of E. coli that are auxotrophic for all 20 amino acids. We were able to find 3 strains auxotrophic for at least two amino acids. So, we thought that we could mate the E. coli cells and get a strain of E. coli auxotrophic for 8 amino acids.

As a result, we need to find and obtain:

What We Need	Status
Y10 E. coli Strain	Found at Yale University, but have not requested a copy Picture of Yale University E.coli database results: Media:Yale.png
58-161 E.coli Strain	Found at Yale University, but have not requested a copy
WWU E. coli Strain	Found at Yale University is call E. coli strain 15 T-, but have not requested a copy
Protocol for mating E. coli	Protocol Options
Protocol for changing a haploid to a diploid	HrF cross will work for mating protocol to get haploid

Mutated Enzymes Causing Auxotroph's for Amino Acids: Media:AminoAcidAuxotrophs.pptx

Sequences of Genes causing Auxtrophs used in strain DL39: Media: aminacidsequences.docx