JP Mar 08 16
materials and methods and early results <- oral presentation #1
Our program won't accept new names.
Made an excel file for the POUF name and its new identity. POUFs not in the list are still unknown. Uploaded this to the wiki.
Plan: look at expression change - from off to on or from on to off ; degree of expression doesn't matter.
Then look at function.
Our potential gene:
-not present in not fed
-upregulated in fed
OR
-only present in not fed -downregulated in fed
Could be: -hormone -transcription factor -receptor
Generated some word files, played with the R code (0.1, 0.1) (mean = 1) etc. first finding:
Contig536_Protein_of_unknown_function_2542
intestine_no_3 1
intestine_no_1 0
intestine_no_2 0
intestine_fed_4 9
intestine_fed_5 26
intestine_fed_6 15
-Not translated
-ncbi blastx PREDICTED: protein jagunal homolog 1-like, partial [Gekko japonicus] ACCESSION XP_015284268
The protein encoded by this gene is a transmembrane protein. It functions in the early secretory pathway and is necessary for neutrophil differentiation and survival. Mutations in this gene result in severe congenital neutropenia. [provided by RefSeq, Oct 2014]
