Difference between revisions of "LasR/cI"
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|'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | |'''Step'''|| '''BBa_''' || '''Description''' ||''' Pre-/suffix''' ||''' Enzymes '''||'''Size '''||'''BBa_'''||'''Description'''||'''Pre-/suffix'''||'''Enzymes'''||'''Size'''||'''BBa_'''||'''Size''' | ||
|- | |- | ||
| − | | 1|| | + | | 1|| K091143 ||plasR/cI ||R/X; S/P||S/P || 164+2079||C0261||RBS+luxI ||R/X;S/P ||X/P ||661 ||TBD || |
|- | |- | ||
| 2|| B0015 ||TT ||R/X; S/P||R/X || 129+2079||I13025||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD || | | 2|| B0015 ||TT ||R/X; S/P||R/X || 129+2079||I13025||RBS+Mnt ||R/X;S/P ||R/S ||331 ||TBD || | ||
Revision as of 18:30, 15 July 2008
Goal:
pBAD+RBS+lasR+TT(James DC)
S0388?(I13453+B0034+C0079+B0015)
pLasR/cI+RBS+LuxI+RBS+Mnt+TT
K09110?+C0261(B0034+C0061)+I13025(B0034+C0072)+B0015
Ligation Plan
| Vector | Insert | Product | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Step | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Description | Pre-/suffix | Enzymes | Size | BBa_ | Size |
| 1 | K091143 | plasR/cI | R/X; S/P | S/P | 164+2079 | C0261 | RBS+luxI | R/X;S/P | X/P | 661 | TBD | |
| 2 | B0015 | TT | R/X; S/P | R/X | 129+2079 | I13025 | RBS+Mnt | R/X;S/P | R/S | 331 | TBD | |
| 3 | TBD | RBS+Mnt+TT | R/X;S/P | R/X | TBD | plasR/cI+RBS+luxI | R/X; S/P | R/S | TBD |
Parts
| BBa_ | Description | Source | location('07) | Vector | Responsible |
|---|---|---|---|---|---|
| B0015 | double terminator | registry 07 | Plt.1-1I;Plt.3-3O | pSB1AK3 | |
| I13025 | RBS+Mnt | registry 07 | Plt.1-23J | pSB1A2 | |
| K09110? | plasR/cI | MW | N/A | pSB1A2 | |
| C0261 | RBS+luxI | registry 07 | Plt.1-24A | pSB1A2 |
