LongAmp PCR

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Preparation

1. 0.6uL 10mM dNTPs

2. 1uL 1ng template

3. 1uL 10uM forward primer

4. 1uL 10uM reverse primer

5. 1uL LongAmp Taq Polymerase

6. 4uL LongAmp Buffer

7. 11.4uL dH20


Programed PCR Protocol

Stage 1:

94ºC 5 minutes

Stage 2:

30 cycles

94ºC 30 seconds

60ºC-->45ºC 30 seconds

60ºC 10 minutes

Stage 3:

72ºC 10 minutes

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